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Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus

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  • Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus

    Vet Microbiol. 2017 May;203:143-148. doi: 10.1016/j.vetmic.2017.03.004. Epub 2017 Mar 6.
    Coimmunization with recombinant epitope-expressing baculovirus enhances protective effects of inactivated H5N1 vaccine against heterologous virus.

    Zhang Z1, Zhang J1, Zhang J1, Li Q1, Miao P1, Liu J1, Li S2, Huang J2, Liao M3, Fan H4.
    Author information

    Abstract

    H5N1, a highly pathogenic avian influenza virus (HPAIV), poses a significant threat to poultry and human health. However, currently available inactivated influenza vaccines are less efficacious against viruses that display antigenic drift. In this study, we constructed a recombinant baculovirus (BV-HMNN) expressing four conserved antigen epitopes: H5N1 hemagglutinin stem area amino acids 76-130 (HA2 76-130); three tandem repeats from the ectodomain of the conserved influenza matrix protein M2 (3M2e); nucleoprotein amino acids 55-69 (NP55-69); and nucleoprotein amino acids 380-393 (NP380-393). We evaluated the immunogenicity and protective efficacy of coimmunization with an inactivated avian influenza virus vaccine (Re6) and the recombinant baculovirus (BV-HMNN) against heterologous viral infection in specific-pathogen-free chickens. The chickens immunized with both vaccines (Re6+BV-HMNN) achieved complete protection, was significantly greater than that of chickens vaccinated with Re6 alone. BV-HMNN-supplemented vaccination also reduced viral shedding more effectively than nonsupplemented vaccination. We conclude that coimmunization with both vaccines was superior to immunization with the inactivated vaccine alone in inducing cross-protection against heterologous H5N1 virus.
    Copyright ? 2017. Published by Elsevier B.V.


    KEYWORDS:

    Avian influenza virus; Baculovirus; Epitope; H5N1; Inactivated vaccine

    PMID: 28619136 DOI: 10.1016/j.vetmic.2017.03.004
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