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Sci Rep. 2016 Sep 27;6:33843. doi: 10.1038/srep33843.
Immune Repertoire Diversity Correlated with Mortality in Avian Influenza A (H7N9) Virus Infected Patients.
Hou D1, Ying T2, Wang L2, Chen C1, Lu S3, Wang Q1, Seeley E4, Xu J3, Xi X3, Li T3, Liu J1, Tang X1, Zhang Z3, Zhou J1, Bai C1, Wang C5, Byrne-Steele M5, Qu J6, Han J5, Song Y1,3,7.
Author information
Abstract
Specific changes in immune repertoires at genetic level responding to the lethal H7N9 virus are still poorly understood. We performed deep sequencing on the T and B cells from patients recently infected with H7N9 to explore the correlation between clinical outcomes and immune repertoire alterations. T and B cell repertoires display highly dynamic yet distinct clonotype alterations. During infection, T cell beta chain repertoire continues to contract while the diversity of immunoglobulin heavy chain repertoire recovers. Patient recovery is correlated to the diversity of T cell and B cell repertoires in different ways - higher B cell diversity and lower T cell diversity are found in survivors. The sequences clonally related to known antibodies with binding affinity to H7 hemagglutinin could be identified from survivors. These findings suggest that utilizing deep sequencing may improve prognostication during influenza infection and could help in development of antibody discovery methodologies for the treatment of virus infection.
PMID: 27669665 DOI: 10.1038/srep33843
[PubMed - as supplied by publisher] Free full text
Immune Repertoire Diversity Correlated with Mortality in Avian Influenza A (H7N9) Virus Infected Patients.
Hou D1, Ying T2, Wang L2, Chen C1, Lu S3, Wang Q1, Seeley E4, Xu J3, Xi X3, Li T3, Liu J1, Tang X1, Zhang Z3, Zhou J1, Bai C1, Wang C5, Byrne-Steele M5, Qu J6, Han J5, Song Y1,3,7.
Author information
Abstract
Specific changes in immune repertoires at genetic level responding to the lethal H7N9 virus are still poorly understood. We performed deep sequencing on the T and B cells from patients recently infected with H7N9 to explore the correlation between clinical outcomes and immune repertoire alterations. T and B cell repertoires display highly dynamic yet distinct clonotype alterations. During infection, T cell beta chain repertoire continues to contract while the diversity of immunoglobulin heavy chain repertoire recovers. Patient recovery is correlated to the diversity of T cell and B cell repertoires in different ways - higher B cell diversity and lower T cell diversity are found in survivors. The sequences clonally related to known antibodies with binding affinity to H7 hemagglutinin could be identified from survivors. These findings suggest that utilizing deep sequencing may improve prognostication during influenza infection and could help in development of antibody discovery methodologies for the treatment of virus infection.
PMID: 27669665 DOI: 10.1038/srep33843
[PubMed - as supplied by publisher] Free full text