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Nat Microbiol. 2016 May 23;1(6):16058. doi: 10.1038/nmicrobiol.2016.58.
Selection of antigenically advanced variants of seasonal influenza viruses.
Li C1, Hatta M1, Burke DF2,3, Ping J1, Zhang Y1, Ozawa M1,4, Taft AS1, Das SC1, Hanson AP1, Song J1, Imai M1,5, Wilker PR1, Watanabe T6, Watanabe S6, Ito M7, Iwatsuki-Horimoto K7, Russell CA3,8,9, James SL2,3, Skepner E2,3, Maher EA1, Neumann G1, Klimov AI10, Kelso A11, McCauley J12, Wang D13, Shu Y13, Odagiri T14, Tashiro M14, Xu X10, Wentworth DE10, Katz JM10, Cox NJ10, Smith DJ2,3,15, Kawaoka Y1,4,6,7.
Author information
Abstract
Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014-2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature.
PMID: 27572841 DOI: 10.1038/nmicrobiol.2016.58
[PubMed - in process]
Selection of antigenically advanced variants of seasonal influenza viruses.
Li C1, Hatta M1, Burke DF2,3, Ping J1, Zhang Y1, Ozawa M1,4, Taft AS1, Das SC1, Hanson AP1, Song J1, Imai M1,5, Wilker PR1, Watanabe T6, Watanabe S6, Ito M7, Iwatsuki-Horimoto K7, Russell CA3,8,9, James SL2,3, Skepner E2,3, Maher EA1, Neumann G1, Klimov AI10, Kelso A11, McCauley J12, Wang D13, Shu Y13, Odagiri T14, Tashiro M14, Xu X10, Wentworth DE10, Katz JM10, Cox NJ10, Smith DJ2,3,15, Kawaoka Y1,4,6,7.
Author information
Abstract
Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014-2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature.
PMID: 27572841 DOI: 10.1038/nmicrobiol.2016.58
[PubMed - in process]