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J Infect Dis. 2016 May 30. pii: jiw230. [Epub ahead of print]
Live attenuated influenza vaccination in children induces B-cell responses in tonsils.
Mohn KG1, Brokstad KA2, Pathirana RD1, Bredholt G3, Jul-Larsen ?1, Trieu MC3, Lartey SL3, Montemoli E4, T?ndel C5, Aarstad HJ6, Cox RJ7.
Author information
Abstract
BACKGROUND:
Tonsils play a key role in eliciting immune responses against respiratory pathogens. Little is known about how tonsils contribute to the local immune response after intranasal vaccination. Here, we uniquely report the mucosal humoral responses in tonsils and saliva after intranasal live attenuated influenza vaccine (LAIV) in children.
METHODS:
Blood, saliva and tonsils samples were collected from 39 children pre-and post-LAIV and from 16 age-matched, non-vaccinated controls. Serum antibody responses were determined by Hemagglutination Inhibition(HI). Salivary IgA was measured by ELISA. Antibody secreting(ASC) and memory B(MBC) cellular responses were enumerated in tonsils and blood.
RESULTS:
Significant increases were observed in serum antibodies and salivary IgA to H3N2 and B strains, as early as 14 days post-vaccination, but not to H1N1. Influenza-specific salivary IgA correlated with serum HI responses, making this a new possible indicator of vaccine immunogenicity in children. LAIV augmented influenza-specific B-cellular responses in tonsils and blood. Tonsillar MBC responses correlated with systemic MBC and serological responses. Na?ve children showed significant increases in MBC after LAIV.
CONCLUSION:
This is the first study to demonstrate that LAIV elicits humoral B-cellular responses in tonsils of young children. Furthermore, salivary IgA represents an easy sampling method for measuring immunogenicity after vaccination.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.
PMID: 27247344 [PubMed - as supplied by publisher]
Live attenuated influenza vaccination in children induces B-cell responses in tonsils.
Mohn KG1, Brokstad KA2, Pathirana RD1, Bredholt G3, Jul-Larsen ?1, Trieu MC3, Lartey SL3, Montemoli E4, T?ndel C5, Aarstad HJ6, Cox RJ7.
Author information
Abstract
BACKGROUND:
Tonsils play a key role in eliciting immune responses against respiratory pathogens. Little is known about how tonsils contribute to the local immune response after intranasal vaccination. Here, we uniquely report the mucosal humoral responses in tonsils and saliva after intranasal live attenuated influenza vaccine (LAIV) in children.
METHODS:
Blood, saliva and tonsils samples were collected from 39 children pre-and post-LAIV and from 16 age-matched, non-vaccinated controls. Serum antibody responses were determined by Hemagglutination Inhibition(HI). Salivary IgA was measured by ELISA. Antibody secreting(ASC) and memory B(MBC) cellular responses were enumerated in tonsils and blood.
RESULTS:
Significant increases were observed in serum antibodies and salivary IgA to H3N2 and B strains, as early as 14 days post-vaccination, but not to H1N1. Influenza-specific salivary IgA correlated with serum HI responses, making this a new possible indicator of vaccine immunogenicity in children. LAIV augmented influenza-specific B-cellular responses in tonsils and blood. Tonsillar MBC responses correlated with systemic MBC and serological responses. Na?ve children showed significant increases in MBC after LAIV.
CONCLUSION:
This is the first study to demonstrate that LAIV elicits humoral B-cellular responses in tonsils of young children. Furthermore, salivary IgA represents an easy sampling method for measuring immunogenicity after vaccination.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America.
PMID: 27247344 [PubMed - as supplied by publisher]