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Nanomedicine. 2016 Apr 22. pii: S1549-9634(16)30033-8. doi: 10.1016/j.nano.2016.04.005. [Epub ahead of print]
An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.
Cao W1, Davis WG2, Kim JH2, De La Cruz JA2, Taylor A2, Hendrickson GR3, Kumar A2, Ranjan P2, Lyon LA3, Katz JM2, Gangappa S2, Sambhara S2.
Author information
Abstract
To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine.
Copyright ? 2015. Published by Elsevier Inc.
KEYWORDS:
H5N1 vaccine; adaptive immunity; adjuvant; influenza; innate immunity; oil-in-water nanoemulsion
PMID: 27112307 [PubMed - as supplied by publisher]
An oil-in-water nanoemulsion enhances immunogenicity of H5N1 vaccine in mice.
Cao W1, Davis WG2, Kim JH2, De La Cruz JA2, Taylor A2, Hendrickson GR3, Kumar A2, Ranjan P2, Lyon LA3, Katz JM2, Gangappa S2, Sambhara S2.
Author information
Abstract
To enhance the immunogenicity of the Influenza H5N1 vaccine, we developed an oil-in-water nanoemulsion (NE) adjuvant. NE displayed good temperature stability and maintained particle size. More importantly, it significantly enhanced IL-6 and MCP-1 production to recruit innate cells, including neutrophils, monocytes/macrophages and dendritic cells to the local environment. Furthermore, NE enhanced dendritic cell function to induce robust antigen-specific T and B cell immune responses. NE-adjuvanted H5N1 vaccine not only elicited significantly higher and long-lasting antibody responses, but also conferred enhanced protection against homologous clade 1 as well as heterologous clade 2 H5N1 virus challenge in young as well as in aged mice. The pre-existing immunity to seasonal influenza did not affect the immunogenicity of NE-adjuvanted H5N1 vaccine.
Copyright ? 2015. Published by Elsevier Inc.
KEYWORDS:
H5N1 vaccine; adaptive immunity; adjuvant; influenza; innate immunity; oil-in-water nanoemulsion
PMID: 27112307 [PubMed - as supplied by publisher]