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Virology. 2014 Oct 28;471-473C:93-104. doi: 10.1016/j.virol.2014.10.003. [Epub ahead of print]
Live attenuated influenza A virus vaccine protects against A(H1N1)pdm09 heterologous challenge without vaccine associated enhanced respiratory disease.
Gauger PC1, Loving CL2, Khurana S3, Lorusso A2, Perez DR4, Kehrli ME Jr2, Roth JA5, Golding H3, Vincent AL6.
Author information
Abstract
Live-attenuated influenza virus (LAIV) vaccines may provide cross-protection against contemporary influenza A virus (IAV) in swine. Conversely, whole inactivated virus (WIV) vaccines have the potential risk of vaccine-associated enhanced respiratory disease (VAERD) when challenged with IAV of substantial antigenic drift. A temperature sensitive, intranasal H1N2 LAIV was compared to wild type exposure (WT) and an intramuscular WIV vaccine in a model shown to induce VAERD. WIV vaccinated swine challenged with pandemic A/H1N1 (H1N1pdm09) were not protected from infection and demonstrated severe respiratory disease consistent with VAERD. Lung lesions were mild and challenge virus was not detected in the respiratory tract of LAIV vaccinates. High levels of post-vaccination IgG serum antibodies targeting the H1N1pdm09 HA2 stalk domain were exclusively detected in the WIV group and associated with increased H1N1pdm09 virus infectivity in MDCK cells. In contrast, infection-enhancing antibodies were not detected in the serum of LAIV vaccinates and VAERD was not observed.
Published by Elsevier Inc.
KEYWORDS:
Enhanced pneumonia; Influenza A; Live attenuated influenza virus; Pandemic H1N1; Swine; Whole inactivated virus
PMID:
25461535
[PubMed - as supplied by publisher]
Live attenuated influenza A virus vaccine protects against A(H1N1)pdm09 heterologous challenge without vaccine associated enhanced respiratory disease.
Gauger PC1, Loving CL2, Khurana S3, Lorusso A2, Perez DR4, Kehrli ME Jr2, Roth JA5, Golding H3, Vincent AL6.
Author information
Abstract
Live-attenuated influenza virus (LAIV) vaccines may provide cross-protection against contemporary influenza A virus (IAV) in swine. Conversely, whole inactivated virus (WIV) vaccines have the potential risk of vaccine-associated enhanced respiratory disease (VAERD) when challenged with IAV of substantial antigenic drift. A temperature sensitive, intranasal H1N2 LAIV was compared to wild type exposure (WT) and an intramuscular WIV vaccine in a model shown to induce VAERD. WIV vaccinated swine challenged with pandemic A/H1N1 (H1N1pdm09) were not protected from infection and demonstrated severe respiratory disease consistent with VAERD. Lung lesions were mild and challenge virus was not detected in the respiratory tract of LAIV vaccinates. High levels of post-vaccination IgG serum antibodies targeting the H1N1pdm09 HA2 stalk domain were exclusively detected in the WIV group and associated with increased H1N1pdm09 virus infectivity in MDCK cells. In contrast, infection-enhancing antibodies were not detected in the serum of LAIV vaccinates and VAERD was not observed.
Published by Elsevier Inc.
KEYWORDS:
Enhanced pneumonia; Influenza A; Live attenuated influenza virus; Pandemic H1N1; Swine; Whole inactivated virus
PMID:
25461535
[PubMed - as supplied by publisher]
