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J Med Virol
. 2025 Oct;97(10):e70614.
doi: 10.1002/jmv.70614. Novel Mini-Hemagglutinin Subunit Vaccine Induces Robust Mucosal and Systemic Immunity and Protection Against a Lethal Challenge With the H1N1 and H5N1 Influenza Viruses in Mice
Tangqi Wang 1 2 , Ruiwen Han 1 2 , Jia Li 1 2 , Xueting Cheng 1 , Zhenyong Qi 3 , Jialuo Bing 3 , Chengcheng Zhai 1 , Donghong Wang 1 , Yao Deng 1 , Wenjie Tan 1 2
Affiliations
Conventional influenza vaccines target the globular head domains of hemagglutinins (HAs), which are highly plastic and provide strain-specific immunity. Therefore, a universal influenza vaccine with conserved antigens and novel adjuvants is urgently required. Mucosal immunity is the first line of defense against viral infections; however, proven mucosal adjuvants are lacking. Therefore, we developed an intranasal Mini-HA subunit vaccine combined with the CTA1-DD adjuvant, which elicited robust mucosal and systemic immune responses in the lungs and spleens of mice, including antigen-specific IgA, IgG, and effector T cells. Furthermore, the Mini-HA subunit vaccine provided the mice with cross-protection against H1N1 and H5N1 influenza viruses. These results indicate that combining the Mini-HA antigen and mucosal adjuvant CTA1-DD is a promising strategy for developing broad-spectrum influenza vaccines.
Keywords: CTA1‐DD adjuvant; broad‐spectrum influenza vaccine; hemagglutinin stem; intranasal immunization; mucosal immunity; systemic immunity.
. 2025 Oct;97(10):e70614.
doi: 10.1002/jmv.70614. Novel Mini-Hemagglutinin Subunit Vaccine Induces Robust Mucosal and Systemic Immunity and Protection Against a Lethal Challenge With the H1N1 and H5N1 Influenza Viruses in Mice
Tangqi Wang 1 2 , Ruiwen Han 1 2 , Jia Li 1 2 , Xueting Cheng 1 , Zhenyong Qi 3 , Jialuo Bing 3 , Chengcheng Zhai 1 , Donghong Wang 1 , Yao Deng 1 , Wenjie Tan 1 2
Affiliations
- PMID: 40985326
- DOI: 10.1002/jmv.70614
Conventional influenza vaccines target the globular head domains of hemagglutinins (HAs), which are highly plastic and provide strain-specific immunity. Therefore, a universal influenza vaccine with conserved antigens and novel adjuvants is urgently required. Mucosal immunity is the first line of defense against viral infections; however, proven mucosal adjuvants are lacking. Therefore, we developed an intranasal Mini-HA subunit vaccine combined with the CTA1-DD adjuvant, which elicited robust mucosal and systemic immune responses in the lungs and spleens of mice, including antigen-specific IgA, IgG, and effector T cells. Furthermore, the Mini-HA subunit vaccine provided the mice with cross-protection against H1N1 and H5N1 influenza viruses. These results indicate that combining the Mini-HA antigen and mucosal adjuvant CTA1-DD is a promising strategy for developing broad-spectrum influenza vaccines.
Keywords: CTA1‐DD adjuvant; broad‐spectrum influenza vaccine; hemagglutinin stem; intranasal immunization; mucosal immunity; systemic immunity.