Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza

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Hum Vaccin. 2011 Jan 1;7. [Epub ahead of print]
Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza.

Shoji Y, Chichester JA, Jones M, Manceva SD, Damon E, Mett V, Musiychuk K, Bi H, Farrance C, Shamloul M, Kushnir N, Sharma S, Yusibov V.

Fraunhofer USA Center for Molecular Biotechnology, Newark, DE USA.
Abstract

In 2009, a novel H1N1 swine influenza virus was isolated from infected humans in Mexico and the United States, and rapidly spread around the world. Another virus, a highly pathogenic avian influenza virus of the H5N1 subtype, identified by the World Health Organization as a potential pandemic threat in 1997, continues to be a significant risk. While vaccination is the preferred strategy for the prevention and control of influenza infections, the traditional egg-based approach to producing influenza vaccines does not provide sufficient capacity and adequate speed to satisfy global needs to combat newly emerging strains, seasonal or potentially pandemic. Significant efforts are underway to develop and implement new cell substrates with improved efficiency for influenza vaccine development and manufacturing. In recent years, plants have been used to produce recombinant proteins including subunit vaccines and antibodies. The main advantages of using plant systems for the production of vaccine antigens against influenza are their independence from pathogenic viruses, and cost and time efficiency. Here, we describe the large-scale production of recombinant hemagglutinin proteins from A/California/04/09 (H1N1) and A/Indonesia/05/05 (H5N1) strains of influenza virus in Nicotiana benthamiana plants, and their immunogenicity (serum hemagglutination inhibition and virus neutralizing antibodies), and safety in animal models. These results support the testing of these candidate vaccines in human volunteers and also the utility of our plant expression system for large-scale recombinant influenza vaccine production.

PMID: 21266846 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/pubmed/21266846

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Re: Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza

See this open source article from 2010 for a summary of the potential for plant based vaccine production in developing countries. link


Developments in Plant-Based Vaccines Against Diseases of Concern in Developing Countries (2010)

Evangelina G?mez, Silvina Chimeno Zoth, Elisa Carrillo and Anal?a Berinstein

Abstract: Vaccination has been extensively used to prevent, eradicate and control infectious diseases. However, the development and implementation of new vaccines remains prohibitively expensive for middle and low income countries where such measures are surely needed most. The production of plant-based vaccines provides a promising alternative to create affordable biological products. In the past decade, a growing number of research groups worldwide have studied plant expression and parenteral or oral delivery of vaccine antigens, some showing promising potential. Here, we describe the basis of the system and the state of the art of developments regarding diseases of concern in developing countries.