Nature . Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses

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Nature


. 2021 May 10.
doi: 10.1038/s41586-021-03594-0. Online ahead of print.
Neutralizing antibody vaccine for pandemic and pre-emergent coronaviruses


Kevin O Saunders ^{1 2 3 4} , Esther Lee ^{5 6} , Robert Parks ^{5 6} , David R Martinez ⁷ , Dapeng Li ^{5 6} , Haiyan Chen ^{5 6} , Robert J Edwards ^{5 6} , Sophie Gobeil ^{5 6} , Maggie Barr ^{5 6} , Katayoun Mansouri ^{5 6} , S Munir Alam ^{5 6} , Laura L Sutherland ^{5 6} , Fangping Cai ^{5 6} , Aja M Sanzone ^{5 6} , Madison Berry ^{5 6} , Kartik Manne ^{5 6} , Kevin W Bock ⁸ , Mahnaz Minai ⁸ , Bianca M Nagata ⁸ , Anyway B Kapingidza ^{5 6} , Mihai Azoitei ^{5 6} , Longping V Tse ⁷ , Trevor D Scobey ⁷ , Rachel L Spreng ^{5 6} , R Wes Rountree ^{5 6} , C Todd DeMarco ^{5 6} , Thomas N Denny ^{5 6} , Christopher W Woods ^{5 6 9} , Elizabeth W Petzold ⁹ , Juanjie Tang ¹⁰ , Thomas H Oguin 3rd ^{5 6} , Gregory D Sempowski ^{5 6} , Matthew Gagne ¹¹ , Daniel C Douek ¹¹ , Mark A Tomai ¹² , Christopher B Fox ¹³ , Robert Seder ¹¹ , Kevin Wiehe ^{5 6} , Drew Weissman ¹⁴ , Norbert Pardi ¹⁴ , Hana Golding ¹⁰ , Surender Khurana ¹⁰ , Priyamvada Acharya ^{5 15} , Hanne Andersen ¹⁶ , Mark G Lewis ¹⁶ , Ian N Moore ⁸ , David C Montefiori ^{5 15} , Ralph S Baric ⁷ , Barton F Haynes ^{17 18 19}



Affiliations

• PMID: 33971664
• DOI: 10.1038/s41586-021-03594-0

Abstract

Betacoronaviruses (betaCoVs) caused the severe acute respiratory syndrome (SARS) and Middle East Respiratory Syndrome (MERS) outbreaks, and the SARS-CoV-2 pandemic^1-4. Vaccines that elicit protective immunity against SARS-CoV-2 and betaCoVs circulating in animals have the potential to prevent future betaCoV pandemics. Here, we show that macaque immunization with a multimeric SARS-CoV-2 receptor binding domain (RBD) nanoparticle adjuvanted with 3M-052/Alum elicited cross-neutralizing antibody (cross-nAb) responses against batCoVs, SARS-CoV-1, SARS-CoV-2, and SARS-CoV-2 variants B.1.1.7, P.1, and B.1.351. Nanoparticle vaccination resulted in a SARS-CoV-2 reciprocal geometric mean neutralization ID50 titer of 47,216, and protection against SARS-CoV-2 in macaque upper and lower respiratory tracts. Importantly, nucleoside-modified mRNA encoding a stabilized transmembrane spike or monomeric RBD also induced SARS-CoV-1 and batCoV cross-nAbs, albeit at lower titers. These results demonstrate current mRNA vaccines may provide some protection from future zoonotic betaCoV outbreaks, and provide a platform for further development of pan-betaCoV vaccines.