Announcement

Collapse
No announcement yet.

Virol J . Novel neutralizing SARS-CoV-2-specific mAbs offer detection of RBD linear epitopes

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Virol J . Novel neutralizing SARS-CoV-2-specific mAbs offer detection of RBD linear epitopes

    Virol J


    . 2024 Feb 6;21(1):37.
    doi: 10.1186/s12985-024-02304-2. Novel neutralizing SARS-CoV-2-specific mAbs offer detection of RBD linear epitopes

    Seyed Mostafa Mostafavi Zadeh 1 2 , Ali Ahmad Bayat 3 , Hosein Shahsavarani 4 5 , Feridoun Karimi-Busheri 6 , Jafar Kiani 7 8 , Roya Ghods 9 10 , Zahra Madjd 11 12



    AffiliationsFree article Abstract

    Background: To stop the spread of the COVID-19 disease, it is crucial to create molecular tools to investigate and diagnose COVID-19. Current efforts focus on developing specific neutralizing monoclonal antibodies (NmAbs) elicited against the receptor-binding domain (RBD).
    Methods: In the present study, recombinant RBD (rRBD) protein was produced in E. coli, followed by immunizing mice with purified rRBD. ELISA was applied to screen the hybridomas for positive reactivity with rRBD protein. The linear and conformational epitopes of the mAbs were subsequently identified using western blot. Finally, the reactivity, affinity, and neutralization activity of the purified mAbs were evaluated using ELISA.
    Results: All mAbs exhibited similar reactivity trends towards both eukaryotic RBD and prokaryotic rRBD in ELISA. Among them, 2E7-D2 and 2B4-G8 mAbs demonstrated higher reactivity than other mAbs. Additionally, in western blot assays, these two mAbs could detect reducing and non-reducing rRBD, indicating recognition of linear epitopes. Notably, five mAbs effectively blocked rRBD- angiotensin-converting enzyme 2 (ACE2) interaction, while two high-affinity mAbs exhibited potent neutralizing activity against eukaryotic RBD.
    Conclusion: In the current study, we generated and characterized new RBD-specific mAbs using the hybridoma technique that recognized linear and conformational epitopes in RBD with neutralization potency. Our mAbs are novel candidates for diagnosing and treating SARS-CoV-2.

    Keywords: Linear epitope; Monoclonal antibody; Neutralizing antibody; RBD; SARS-CoV-2.

    ‚Äč
Working...
X