Viruses . Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2

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Viruses


. 2020 Jun 28;12(7):E699.
doi: 10.3390/v12070699.
Saxifraga spinulosa-Derived Components Rapidly Inactivate Multiple Viruses Including SARS-CoV-2

Yohei Takeda ¹ , Toshihiro Murata ² , Dulamjav Jamsransuren ³ , Keisuke Suganuma ^{1 4} , Yuta Kazami ³ , Javzan Batkhuu ⁵ , Duger Badral ⁶ , Haruko Ogawa ³


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• PMID: 32605306
• DOI: 10.3390/v12070699

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Abstract

Novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A virus (IAV), and norovirus (NV) are highly contagious pathogens that threaten human health. Here we focused on the antiviral potential of the medicinal herb, Saxifraga spinulosa (SS). Water-soluble extracts of SS were prepared, and their virus-inactivating activity was evaluated against the human virus pathogens SARS-CoV-2 and IAV; we also examined virucidal activity against feline calicivirus and murine norovirus, which are surrogates for human NV. Among our findings, we found that SS-derived gallocatechin gallate compounds were capable of inactivating all viruses tested. Interestingly, a pyrogallol-enriched fraction (Fr 1C) inactivated all viruses more rapidly and effectively than did any of the component compounds used alone. We found that 25 ?g/mL of Fr 1C inactivated >99.6% of SARS-CoV-2 within 10 s (reduction of ≥2.33 log₁₀ TCID₅₀/mL). Fr 1C resulted in the disruption of viral genomes and proteins as determined by gel electrophoresis, electron microscopy, and reverse transcription-PCR. Taken together, our results reveal the potential of Fr 1C for development as a novel antiviral disinfectant.

Keywords: SARS-CoV-2; Saxifraga spinulosa; medicinal herb; pandemic; pyrogallol-enriched fraction; virus disinfectant.