Curr Protoc Microbiol . An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening

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Curr Protoc Microbiol


. 2020 Sep;58(1):e108.
doi: 10.1002/cpmc.108.
An In Vitro Microneutralization Assay for SARS-CoV-2 Serology and Drug Screening


Fatima Amanat ^{1 2} , Kris M White ^{2 3} , Lisa Miorin ^{2 3} , Shirin Strohmeier ² , Meagan McMahon ² , Philip Meade ^{1 2} , Wen-Chun Liu ^{2 3} , Randy A Albrecht ^{2 3} , Viviana Simon ^{2 3 4} , Luis Martinez-Sobrido ⁵ , Thomas Moran ² , Adolfo Garc?a-Sastre ^{2 3 4 6} , Florian Krammer ²



Affiliations

• PMID: 32585083
• DOI: 10.1002/cpmc.108

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in the city of Wuhan, Hubei Province, China, in late 2019. Since then, the virus has spread globally and caused a pandemic. Assays that can measure the antiviral activity of antibodies or antiviral compounds are needed for SARS-CoV-2 vaccine and drug development. Here, we describe in detail a microneutralization assay, which can be used to assess in a quantitative manner if antibodies or drugs can block entry and/or replication of SARS-CoV-2 in vitro. ? 2020 Wiley Periodicals LLC. Basic Protocol 1: Microneutralization assay to test inhibition of virus by antibodies (purified antibodies or serum/plasma) Basic Protocol 2: Screening of anti-SARS-CoV-2 compounds in vitro Support Protocol: SARS-CoV-2 propagation.

Keywords: COVID-19; COVID19; SARS-CoV-2; antivirals; medium-throughput screening; microneutralization; neutralization.