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MAbs . Potent Neutralization of SARS-CoV-2 by Human Antibody Heavy-Chain Variable Domains Isolated From a Large Library With a New Stable Scaffold

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  • MAbs . Potent Neutralization of SARS-CoV-2 by Human Antibody Heavy-Chain Variable Domains Isolated From a Large Library With a New Stable Scaffold


    MAbs


    . 2020 Jan 1;12(1):1778435.
    doi: 10.1080/19420862.2020.1778435.
    Potent Neutralization of SARS-CoV-2 by Human Antibody Heavy-Chain Variable Domains Isolated From a Large Library With a New Stable Scaffold


    Zehua Sun 1 , Chuan Chen 1 , Wei Li 1 , David R Martinez 2 , Aleksandra Drelich 3 , Du-San Baek 1 , Xianglei Liu 1 , John W Mellors 1 4 , Chien-Te Tseng 3 , Ralph S Baric 2 , Dimiter S Dimitrov 1 4



    Affiliations

    Abstract

    Effective therapies are urgently needed for COVID-19. Here we describe the identification of a new stable human immunoglobulin G1 heavy-chain variable (VH) domain scaffold that was used for the construction of a large library, lCAT6, of engineered human VHs. This library was panned against the receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) glycoprotein. Two VH domains (VH ab6 and VH m397) were selected and fused to Fc for increased half-life in circulation. The VH-Fc ab6 and m397 specifically neutralized SARS-CoV-2 with high potencies (50% neutralization at 0.35 ?g/ml and 1.5 ?g/ml, respectively) as measured by two independent replication-competent virus neutralization assays. Ab6 and m397 competed with ACE2 for binding to RBD, suggesting a competitive mechanism of virus neutralization. These VH domains may have potential applications for prophylaxis and therapy of COVID-19 alone or in combination, as well as for diagnosis and as tools for research.

    Keywords: SARS-CoV-2; Therapeutic antibodies; coronaviruses.

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