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Pharmacol Res. Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2)

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  • Pharmacol Res. Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2)


    Pharmacol Res. 2020 Mar 20:104761. doi: 10.1016/j.phrs.2020.104761. [Epub ahead of print]
    Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2).


    Runfeng L1, Yunlong H2, Jicheng H3, Weiqi P1, Qinhai M1, Yongxia S3, Chufang L1, Jin Z1, Zhenhua J2, Haiming J1, Kui Z3, Shuxiang H3, Jun D3, Xiaobo L3, Xiaotao H4, Lin W4, Nanshan Z1, Zifeng Y5.

    Author information




    Abstract

    PURPOSE:

    Lianhuaqingwen (LH) as traditional Chinese medicine (TCM) formula has been used to treat influenza and exerted broad-spectrum antiviral effects on a series of influenza viruses and immune regulatory effects [1]. The goal of this study is to demonstrate the antiviral activity of LH against the novel SARS-CoV-2 virus and its potential effect in regulating host immune response.
    METHODS:

    The antiviral activity of LH against SARS-CoV-2 was assessed in Vero E6 cells using CPE and plaque reduction assay. The effect of LH on virion morphology was visualized under transmission electron microscope. Pro-inflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays.
    RESULTS:

    LH significantly inhibited SARS-CoV-2 replication in Vero E6 cells and markedly reduced pro-inflammatory cytokines (TNF-α, IL-6, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Furthermore, LH treatment resulted in abnormal particle morphology of virion in cells.
    CONCLUSIONS:

    LH significantly inhibits the SARS-COV-2 replication, affects virus morphology and exerts anti-inflammatory activity in vitro. These findings indicate that LH protects against the virus attack, making its use a novel strategy for controlling the COVID-19 disease.
    Copyright ? 2020. Published by Elsevier Ltd.



    KEYWORDS:

    Lianhuaqingwen; SARS-CoV-2; anti-inflammatory; coronavirus


    PMID:32205232DOI:10.1016/j.phrs.2020.104761

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