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J Biomed Sci . A structure and knowledge-based combinatorial approach to engineering universal scFv antibodies against influenza M2 protein

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  • J Biomed Sci . A structure and knowledge-based combinatorial approach to engineering universal scFv antibodies against influenza M2 protein

    J Biomed Sci


    . 2023 Jul 25;30(1):56.
    doi: 10.1186/s12929-023-00950-2. A structure and knowledge-based combinatorial approach to engineering universal scFv antibodies against influenza M2 protein

    Ujjwal Kumar 1 , Priya Goyal 2 , Zaid K Madni 1 , Kajal Kamble 3 , Vineet Gaur 4 , Maitreyi S Rajala 2 , Dinakar M Salunke 5



    AffiliationsAbstract

    Background: The influenza virus enters the host via hemagglutinin protein binding to cell surface sialic acid. Receptor-mediated endocytosis is followed by viral nucleocapsid uncoating for replication aided by the transmembrane viral M2 proton ion channel. M2 ectodomain (M2e) is a potential universal candidate for monoclonal antibody therapy owing to its conserved nature across influenza virus subtypes and its importance in viral propagation.
    Methods: The phage-displayed naive human antibody libraries were screened against the short stretch of the N-terminal 10-mer peptide (SLLTEVETPI) of the M2e. ELISA, BLI, and flow cytometry assays were used to examine scFv binding to M2e epitopes. The scFv crystal structures were determined to examine the nature of the interactions. The potencies of the scFvs against the influenza virus were demonstrated by real-time PCR and confocal microscopy imaging.
    Results: The four unique scFv clones were obtained from the scFv phage-display antibody libraries and shown to exhibit binding with the 10-mer conserved part of the M2e and with full-length M2 protein expressed on the HEK293T cells. The crystal structure of scFv AU1 with M2e peptide showed the peptide as a dimer in the parallel beta-sheet conformation bound at the interface of two scFv CDRs. The scFv AU1 significantly restricted the release of H1N1 virus progeny from the infected A549 cells.
    Conclusion: This structural and biochemical study showcased the binding of antibody scFv molecules with M2e peptide dimer, providing the structural insights for the function effect in terms of recognizing and restricting the release of new viral particles from an infected host cell.

    Keywords: Co-crystallization; Influenza virus; M2 ion channel protein and M2e dimerization; Naive human antibody scFv library; Virus regression.




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