Development and validation of an LC-MS/MS method for the quantitative analysis of the anti-influenza agent camphecene in rat plasma and its application to study the blood-to-plasma distribution of the agent

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J Pharm Biomed Anal. 2019 Dec 10;180:113039. doi: 10.1016/j.jpba.2019.113039. [Epub ahead of print] Development and validation of an LC-MS/MS method for the quantitative analysis of the anti-influenza agent camphecene in rat plasma and its application to study the blood-to-plasma distribution of the agent.

Okhina AA¹, Rogachev AD², Yarovaya OI¹, Khvostov MV¹, Tolstikova TG¹, Pokrovsky AG³, Khazanov VA⁴, Salakhutdinov NF¹.
Author information

1 N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogov Str., 2, Novosibirsk 630090, Russia. 2 N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogov Str., 2, Novosibirsk 630090, Russia. Electronic address: rogachev@nioch.nsc.ru. 3 Novosibirsk State University, Pirogov Str., 2, Novosibirsk 630090, Russia. 4 Innovative Pharmacology Research, LLC (IPHAR), Elizarovikh Str., 79/4, Tomsk 634021, Russia.

Abstract

A method of quantitative determination of camphecene, a new anti-influenza agent, in rat blood plasma based on LC-MS/MS was developed, validated and used to study the distribution of the agent between blood cells and blood plasma. The method was validated according to FDA and EMA recommendations in terms of selectivity, linearity, accuracy, precision, recovery, stability and carry-over. Plasma samples were precipitated with methanol followed by the addition of a methanolic solution of 2-adamantylamine hydrochloride (internal standard). HPLC analysis was performed on a reversed-phase column; the total time of analysis was 11 min, including column equilibration. MS/MS detection was performed on a 3200 QTRAP triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Transitions 196.4 → 122.2/153.3 and 152.2 → 93.1/107.2 were monitored for camphecene and the internal standard, respectively. The calibration curve was built in the concentration range of 10-5000 ng/ml. The intra-day and inter-day accuracy and precision, carry-over and recovery were within the acceptable limits. It was found that, after spiking blood with camphecene and separating plasma, the concentration of the substance in the latter was close to its initial concentration in the blood. This property of the substance may be useful for clinical trials of the agent. It has also been established that the process of camphecene distribution (adsorption) between blood cells and blood plasma is reversible, and the amount of adsorbed substance is linearly dependent on its initial concentration in the blood for a wide range of concentrations, temperatures and hematocrit values.
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KEYWORDS:

Anti-influenza; Blood-to-plasma distribution; Camphecene; LC-MS/MS

PMID: 31865208 DOI: 10.1016/j.jpba.2019.113039