Tweet
Int Immunol. 2019 Aug 1. pii: dxz054. doi: 10.1093/intimm/dxz054. [Epub ahead of print]
Broad and systemic immune-modulating capacity of plant-derived dsRNA.
Hajake T1,2, Matsuno K3, M Kasumba D1,2, Oda H1,2, Kobayashi M1, Miyata N1,2, Shinji M1,2, Kogure A1,2, Kasajima N3, Okamatsu M4, Sakoda Y3,4, Kato H1,2,5, Fujita T1,2.
Author information
Abstract
Double-stranded RNA (dsRNA) is well characterized as an inducer of antiviral interferon responses. We previously reported that dsRNA extracted from a specific edible plant possesses immune-modulating capacity to confer mice the resistance against respiratory viruses, including the H1N1 strain of the Influenza A virus. We report that the systemic immune-activating capacity of the plant-derived dsRNA protected mice from infection by a highly virulent H5N1 strain of the Influenza A virus. In addition, subcutaneous inoculation of the dsRNA together with inactivated virion of the H5N1 strain of the Influenza A virus suppressed the lethality by the viral infection as compared with individual inoculation of either dsRNA or HA protein, suggesting its potential usage as a vaccination adjuvant. Moreover, intraperitoneal inoculation of the dsRNA limited the growth of B16-F10 melanoma cells through the activation of NK cells in murine models. Taken together, this study demonstrated the systemic immune-modulating capacity of a plant-derived dsRNA and its potential for nucleic acid-based clinical applications.
? The Japanese Society for Immunology. 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
KEYWORDS:
adjuvant; antiviral; double-stranded RNA; melanoma; type I interferon
PMID: 31367737 DOI: 10.1093/intimm/dxz054
Broad and systemic immune-modulating capacity of plant-derived dsRNA.
Hajake T1,2, Matsuno K3, M Kasumba D1,2, Oda H1,2, Kobayashi M1, Miyata N1,2, Shinji M1,2, Kogure A1,2, Kasajima N3, Okamatsu M4, Sakoda Y3,4, Kato H1,2,5, Fujita T1,2.
Author information
Abstract
Double-stranded RNA (dsRNA) is well characterized as an inducer of antiviral interferon responses. We previously reported that dsRNA extracted from a specific edible plant possesses immune-modulating capacity to confer mice the resistance against respiratory viruses, including the H1N1 strain of the Influenza A virus. We report that the systemic immune-activating capacity of the plant-derived dsRNA protected mice from infection by a highly virulent H5N1 strain of the Influenza A virus. In addition, subcutaneous inoculation of the dsRNA together with inactivated virion of the H5N1 strain of the Influenza A virus suppressed the lethality by the viral infection as compared with individual inoculation of either dsRNA or HA protein, suggesting its potential usage as a vaccination adjuvant. Moreover, intraperitoneal inoculation of the dsRNA limited the growth of B16-F10 melanoma cells through the activation of NK cells in murine models. Taken together, this study demonstrated the systemic immune-modulating capacity of a plant-derived dsRNA and its potential for nucleic acid-based clinical applications.
? The Japanese Society for Immunology. 2019. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
KEYWORDS:
adjuvant; antiviral; double-stranded RNA; melanoma; type I interferon
PMID: 31367737 DOI: 10.1093/intimm/dxz054