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PLoS One. 2017 Apr 5;12(4):e0174523. doi: 10.1371/journal.pone.0174523. eCollection 2017.
In vivo inhibition of influenza A virus replication by RNA interference targeting the PB2 subunit via intratracheal delivery.
Huang DT1,2, Lu CY3, Shao PL3, Chang LY2,3, Wang JY1, Chang YH3, Lai MJ3, Chi YH4, Huang LM2,3.
Author information
Abstract
BACKGROUND:
Influenza virus infection is a major threat to human health. Small interfering RNA (siRNA) is a promising approach for the prevention and treatment of viral infections. In this study, we constructed a series of DNA vector-based short hairpin RNAs (shRNAs) that target various genes of the influenza A virus using the polymerase III U6-RNA promoter to prevent influenza virus infection in vitro and in a mouse model.
RESULTS:
Three sets of DNA vector-based shRNA, two targeting genes encoding the polymerase acidic protein (PA) and one targeting polymerase basic protein 2 (PB2), efficiently inhibited the replication of influenza virus A/WSN/33(H1N1) in vitro. We also successfully prevented influenza virus A/WSN/33(H1N1) infection in a C57BL/6 mouse model by intratracheal delivery of anti-PB2 shRNA.
CONCLUSIONS:
Our findings suggest that the PB2-targeting shRNA plasmid showed potential for use as an RNAi-based therapeutic for influenza virus infection.
PMID: 28380007 DOI: 10.1371/journal.pone.0174523
In vivo inhibition of influenza A virus replication by RNA interference targeting the PB2 subunit via intratracheal delivery.
Huang DT1,2, Lu CY3, Shao PL3, Chang LY2,3, Wang JY1, Chang YH3, Lai MJ3, Chi YH4, Huang LM2,3.
Author information
Abstract
BACKGROUND:
Influenza virus infection is a major threat to human health. Small interfering RNA (siRNA) is a promising approach for the prevention and treatment of viral infections. In this study, we constructed a series of DNA vector-based short hairpin RNAs (shRNAs) that target various genes of the influenza A virus using the polymerase III U6-RNA promoter to prevent influenza virus infection in vitro and in a mouse model.
RESULTS:
Three sets of DNA vector-based shRNA, two targeting genes encoding the polymerase acidic protein (PA) and one targeting polymerase basic protein 2 (PB2), efficiently inhibited the replication of influenza virus A/WSN/33(H1N1) in vitro. We also successfully prevented influenza virus A/WSN/33(H1N1) infection in a C57BL/6 mouse model by intratracheal delivery of anti-PB2 shRNA.
CONCLUSIONS:
Our findings suggest that the PB2-targeting shRNA plasmid showed potential for use as an RNAi-based therapeutic for influenza virus infection.
PMID: 28380007 DOI: 10.1371/journal.pone.0174523