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Antivir Ther. 2017 Mar 8. doi: 10.3851/IMP3152. [Epub ahead of print]
Geniposide demonstrates anti-inflammatory and antiviral activity against pandemic A/Jiangsu/1/2009 (H1N1) influenza virus infection in vitro and in vivo.
Yunshi Z1, Jing Y1, Xian Q2, Xing L1, Xieqin L1, Ganzhu F1.
Author information
Abstract
BACKGROUND:
Influenza A viruses (IAVs) remains to be a great threat to human health for centuries, without effective control. Geniposide, a main iridoid glycoside compound extracted from Gardenia jasminoides Ellis (GJ) fruit, possesses various biologic activities including anti-inflammation and anti-virus.
METHODS:
Madin-Darby Canine Kidney (MDCK) cells were infected with pandemic A/Jiangsu/1/2009 (H1N1) influenza virus in vitro. Cytotoxicity and antiviral activity of geniposide were estimated by MTT assay. The influenza respiratory tract infection murine model was established by intranasal instillation of pandemic A/Jiangsu/1/2009 (H1N1) influenza virus. One day after infection, the mice were administered with geniposide (5, 10 or 20 mg/kg/d) or the neuraminidase inhibitor (NAI) peramivir (30 mg/kg/d). Body weight, survival time, viral titer and lung index of the mice were measured. The sandwich enzyme-linked immunosorbent assay (ELISA) was used to examine levels of inflammatory cytokines.
RESULTS:
The data showed that geniposide had little cytotoxicity on MDCK cells and protected them from pandemic A/Jiangsu/1/2009 (H1N1) influenza virus-induced cell injury. In the infected mice, geniposide treatment significantly restored the body weights, decreased the mortality, alleviated viral titers and virus-induced lung lesions. Geniposide substantially inhibited the virus-induced alveolar wall changes, alveolar haemorrhage and neutrophi-infiltration in lung tissues. Levels of inflammatory mediators, including tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-6 (IL-6) and interleukin-10 (IL-10) were also markedly altered after treatment with geniposide.
CONCLUSIONS:
Our investigation suggested that geniposide effectively inhibited cell damage medicated by pandemic A/Jiangsu/1/2009 (H1N1) influenza virus and mitigated virus-induced acute inflammation.
PMID: 28272019 DOI: 10.3851/IMP3152
Geniposide demonstrates anti-inflammatory and antiviral activity against pandemic A/Jiangsu/1/2009 (H1N1) influenza virus infection in vitro and in vivo.
Yunshi Z1, Jing Y1, Xian Q2, Xing L1, Xieqin L1, Ganzhu F1.
Author information
Abstract
BACKGROUND:
Influenza A viruses (IAVs) remains to be a great threat to human health for centuries, without effective control. Geniposide, a main iridoid glycoside compound extracted from Gardenia jasminoides Ellis (GJ) fruit, possesses various biologic activities including anti-inflammation and anti-virus.
METHODS:
Madin-Darby Canine Kidney (MDCK) cells were infected with pandemic A/Jiangsu/1/2009 (H1N1) influenza virus in vitro. Cytotoxicity and antiviral activity of geniposide were estimated by MTT assay. The influenza respiratory tract infection murine model was established by intranasal instillation of pandemic A/Jiangsu/1/2009 (H1N1) influenza virus. One day after infection, the mice were administered with geniposide (5, 10 or 20 mg/kg/d) or the neuraminidase inhibitor (NAI) peramivir (30 mg/kg/d). Body weight, survival time, viral titer and lung index of the mice were measured. The sandwich enzyme-linked immunosorbent assay (ELISA) was used to examine levels of inflammatory cytokines.
RESULTS:
The data showed that geniposide had little cytotoxicity on MDCK cells and protected them from pandemic A/Jiangsu/1/2009 (H1N1) influenza virus-induced cell injury. In the infected mice, geniposide treatment significantly restored the body weights, decreased the mortality, alleviated viral titers and virus-induced lung lesions. Geniposide substantially inhibited the virus-induced alveolar wall changes, alveolar haemorrhage and neutrophi-infiltration in lung tissues. Levels of inflammatory mediators, including tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-6 (IL-6) and interleukin-10 (IL-10) were also markedly altered after treatment with geniposide.
CONCLUSIONS:
Our investigation suggested that geniposide effectively inhibited cell damage medicated by pandemic A/Jiangsu/1/2009 (H1N1) influenza virus and mitigated virus-induced acute inflammation.
PMID: 28272019 DOI: 10.3851/IMP3152