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J Infect Dis. 2009 Aug 15;200(4):492-500.
Viral loads and duration of viral shedding in adult patients hospitalized with influenza.
Lee N, Chan PK, Hui DS, Rainer TH, Wong E, Choi KW, Lui GC, Wong BC, Wong RY, Lam WY, Chu IM, Lai RW, Cockram CS, Sung JJ. Departments of 1Medicine and Therapeutics and 2Microbiology, 3Stanley Ho Centre for Emerging Infectious Diseases, 4Trauma and Emergency Centre, 5Centre for Epidemiology and Biostatistics, The Chinese University of Hong Kong, Hong Kong.
Background.
The goal of this study was to characterize viral loads and factors affecting viral clearance in persons with severe influenza.
Methods.
This was a 1-year prospective, observational study involving consecutive adults hospitalized with influenza. Nasal and throat swabs were collected at presentation, then daily until 1 week after symptom onset. Real-time reverse-transcriptase polymerase chain reaction to determine viral RNA concentration and virus isolation were performed. Viral RNA concentration was analyzed using multiple linear or logistic regressions or mixed-effect models.
Results.
One hundred forty-seven inpatients with influenza A (H3N2) infection were studied (mean age +/- standard deviation, [Formula: see text] years). Viral RNA concentration at presentation positively correlated with symptom scores and was significantly higher than that among time-matched outpatients (control subjects). Patients with major comorbidities had high viral RNA concentration even when presenting >2 days after symptom onset (mean +/- standard deviation, [Formula: see text] vs [Formula: see text] log(10) copies/mL; [Formula: see text]; beta, +0.86 [95% confidence interval, +0.03 to +1.68]). Viral RNA concentration demonstrated a nonlinear decrease with time; 26% of oseltamivir-treated and 57% of untreated patients had RNA detected at 1 week after symptom onset. Oseltamivir started on or before symptom day 4 was independently associated with an accelerated decrease in viral RNA concentration (mean beta [standard error], -1.19 [0.43] and -0.68 [0.33] log(10) copies/mL for patients treated on day 1 and days 2-3, respectively; [Formula: see text]) and viral RNA clearance at 1 week (odds ratio, 0.10 [95% confidence interval, 0.03-0.35] and 0.30 [0.10-0.90] for patients treated on day 1-2 and day 3-4, respectively). Conversely, major comorbidities and systemic corticosteroid use for asthma or chronic obstructive pulmonary disease exacerbations were associated with slower viral clearance. Viral RNA clearance was associated with a shorter hospital stay (7.0 vs 13.5 days; [Formula: see text]).
Conclusion.
Patients hospitalized with severe influenza have more active and prolonged viral replication. Weakened host defenses slow viral clearance, whereas antivirals started within the first 4 days of illness enhance viral clearance.
PMID: 19591575 [PubMed - in proces
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Viral loads and duration of viral shedding in adult patients hospitalized with influenza.
Lee N, Chan PK, Hui DS, Rainer TH, Wong E, Choi KW, Lui GC, Wong BC, Wong RY, Lam WY, Chu IM, Lai RW, Cockram CS, Sung JJ. Departments of 1Medicine and Therapeutics and 2Microbiology, 3Stanley Ho Centre for Emerging Infectious Diseases, 4Trauma and Emergency Centre, 5Centre for Epidemiology and Biostatistics, The Chinese University of Hong Kong, Hong Kong.
Background.
The goal of this study was to characterize viral loads and factors affecting viral clearance in persons with severe influenza.
Methods.
This was a 1-year prospective, observational study involving consecutive adults hospitalized with influenza. Nasal and throat swabs were collected at presentation, then daily until 1 week after symptom onset. Real-time reverse-transcriptase polymerase chain reaction to determine viral RNA concentration and virus isolation were performed. Viral RNA concentration was analyzed using multiple linear or logistic regressions or mixed-effect models.
Results.
One hundred forty-seven inpatients with influenza A (H3N2) infection were studied (mean age +/- standard deviation, [Formula: see text] years). Viral RNA concentration at presentation positively correlated with symptom scores and was significantly higher than that among time-matched outpatients (control subjects). Patients with major comorbidities had high viral RNA concentration even when presenting >2 days after symptom onset (mean +/- standard deviation, [Formula: see text] vs [Formula: see text] log(10) copies/mL; [Formula: see text]; beta, +0.86 [95% confidence interval, +0.03 to +1.68]). Viral RNA concentration demonstrated a nonlinear decrease with time; 26% of oseltamivir-treated and 57% of untreated patients had RNA detected at 1 week after symptom onset. Oseltamivir started on or before symptom day 4 was independently associated with an accelerated decrease in viral RNA concentration (mean beta [standard error], -1.19 [0.43] and -0.68 [0.33] log(10) copies/mL for patients treated on day 1 and days 2-3, respectively; [Formula: see text]) and viral RNA clearance at 1 week (odds ratio, 0.10 [95% confidence interval, 0.03-0.35] and 0.30 [0.10-0.90] for patients treated on day 1-2 and day 3-4, respectively). Conversely, major comorbidities and systemic corticosteroid use for asthma or chronic obstructive pulmonary disease exacerbations were associated with slower viral clearance. Viral RNA clearance was associated with a shorter hospital stay (7.0 vs 13.5 days; [Formula: see text]).
Conclusion.
Patients hospitalized with severe influenza have more active and prolonged viral replication. Weakened host defenses slow viral clearance, whereas antivirals started within the first 4 days of illness enhance viral clearance.
PMID: 19591575 [PubMed - in proces
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