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Int Immunopharmacol. 2013 Nov 4. pii: S1567-5769(13)00400-1. doi: 10.1016/j.intimp.2013.10.020. [Epub ahead of print]
Lactobacillus fermentum CJL-112 protects mice against influenza virus infection by activating T-helper 1 and eliciting a protective immune response.
Yeo JM, Lee HJ, Kim JW, Lee JB, Park SY, Choi IS, Song CS.
Source
CJ Research Institute of Biotechnology, 92-1, Gayang-dong, Gangseo-gu, Seoul 157-724, South Korea. Electronic address: yjmin1177@naver.com.
Abstract
We have previously reported that nasally administered Lactobacillus fermentum CJL-112 (CJL-112) efficiently improves resistance against lethal influenza infection in both mice and chicken. The aim of the present study was to understand the underlying mechanisms of the significant anti-influenza activity of this lactobacilli strain. In vitro, co-culturing of the chicken macrophage cell line HD-11 with CJL-112 significantly increased nitric oxide (NO) production. In vivo, CJL-112 was nasally administered to BALB/c mice for 21days prior to influenza A/NWS/33 (H1N1) virus (IFV) infection. Significant up-regulation of T-helper 1 (Th1) cytokines (IL-2, IFN-γ) was observed, while the levels of T-helper 2 (Th2) cytokines (IL-4, IL-5, IL-10) was either reduced or unchanged than that in control mice were. Furthermore, IgA and specific anti-influenza IgA levels increased significantly in the treated mice than those in untreated mice. Therefore, CJL-112 likely protects the mice against lethal IFV infection via stimulation of macrophages, activation of Th1 and augmentation of IgA production, when directly delivered into the respiratory tract.
? 2013.
KEYWORDS:
IgA, Influenza virus, Lactobacillus fermentum CJL-112, Nitric oxide production, Th1 immune response
PMID:
24201084
[PubMed - as supplied by publisher]
Lactobacillus fermentum CJL-112 protects mice against influenza virus infection by activating T-helper 1 and eliciting a protective immune response.
Yeo JM, Lee HJ, Kim JW, Lee JB, Park SY, Choi IS, Song CS.
Source
CJ Research Institute of Biotechnology, 92-1, Gayang-dong, Gangseo-gu, Seoul 157-724, South Korea. Electronic address: yjmin1177@naver.com.
Abstract
We have previously reported that nasally administered Lactobacillus fermentum CJL-112 (CJL-112) efficiently improves resistance against lethal influenza infection in both mice and chicken. The aim of the present study was to understand the underlying mechanisms of the significant anti-influenza activity of this lactobacilli strain. In vitro, co-culturing of the chicken macrophage cell line HD-11 with CJL-112 significantly increased nitric oxide (NO) production. In vivo, CJL-112 was nasally administered to BALB/c mice for 21days prior to influenza A/NWS/33 (H1N1) virus (IFV) infection. Significant up-regulation of T-helper 1 (Th1) cytokines (IL-2, IFN-γ) was observed, while the levels of T-helper 2 (Th2) cytokines (IL-4, IL-5, IL-10) was either reduced or unchanged than that in control mice were. Furthermore, IgA and specific anti-influenza IgA levels increased significantly in the treated mice than those in untreated mice. Therefore, CJL-112 likely protects the mice against lethal IFV infection via stimulation of macrophages, activation of Th1 and augmentation of IgA production, when directly delivered into the respiratory tract.
? 2013.
KEYWORDS:
IgA, Influenza virus, Lactobacillus fermentum CJL-112, Nitric oxide production, Th1 immune response
PMID:
24201084
[PubMed - as supplied by publisher]
