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J Pharm Biomed Anal. 2013 Oct 14;88C:602-608. doi: 10.1016/j.jpba.2013.10.006. [Epub ahead of print]
New method of screening allergenic components from Shuanghuanglian injection: With RBL-2H3/CMC model online HPLC/MS system.
Han S, Zhang T, Huang J, Cui R, He L.
Source
School of Medicine, Xi'an Jiaotong University, 76#, Yantan Westroad, Xi'an 710061, China.
Abstract
Shuanghuanglian (SHL) injection is a traditional Chinese medicine (TCM) injection widely used in China to treat influenza, tonsillitis, bronchitis, and faucitis. Adverse drug reactions (ADR) of SHL injection, majorly manifested as allergic reactions, were among the leading causes of death from TCM injection. In this study, an RBL-2H3/CMC online LC/MS system was established to screen and identify allergenic components in SHL injection, by which Baicalin was identified as a potential allergenic component. Allergenic activities of baicalin were investigated in RBL-2H3 cell degranulation and β-hexosaminidase release tests in vitro. Our results showed that baicalin dose-dependently induced RBL-2H3 cell degranulation and β-hexosaminidase release in the range of 0-50μg/ml. The RBL-2H3/CMC online LC/MS system developed in this study may potentially be used to screen allergenic components in other TCM injections.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Anaphylactoid reaction, Cell degranulation, Liquid chromatography tandem mass spectrum, RBL-2H3 cell membrane chromatography, β-Hexosaminidase release
PMID:
24211723
[PubMed - as supplied by publisher]
New method of screening allergenic components from Shuanghuanglian injection: With RBL-2H3/CMC model online HPLC/MS system.
Han S, Zhang T, Huang J, Cui R, He L.
Source
School of Medicine, Xi'an Jiaotong University, 76#, Yantan Westroad, Xi'an 710061, China.
Abstract
Shuanghuanglian (SHL) injection is a traditional Chinese medicine (TCM) injection widely used in China to treat influenza, tonsillitis, bronchitis, and faucitis. Adverse drug reactions (ADR) of SHL injection, majorly manifested as allergic reactions, were among the leading causes of death from TCM injection. In this study, an RBL-2H3/CMC online LC/MS system was established to screen and identify allergenic components in SHL injection, by which Baicalin was identified as a potential allergenic component. Allergenic activities of baicalin were investigated in RBL-2H3 cell degranulation and β-hexosaminidase release tests in vitro. Our results showed that baicalin dose-dependently induced RBL-2H3 cell degranulation and β-hexosaminidase release in the range of 0-50μg/ml. The RBL-2H3/CMC online LC/MS system developed in this study may potentially be used to screen allergenic components in other TCM injections.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Anaphylactoid reaction, Cell degranulation, Liquid chromatography tandem mass spectrum, RBL-2H3 cell membrane chromatography, β-Hexosaminidase release
PMID:
24211723
[PubMed - as supplied by publisher]
