Mucosal Immunol . Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells

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Mucosal Immunol


. 2022 Feb 10.
doi: 10.1038/s41385-021-00478-4. Online ahead of print.
Spatial, temporal and molecular dynamics of swine influenza virus-specific CD8 tissue resident memory T cells


Veronica Martini ^{1 2 3} , Matthew Edmans ⁴ , Simon Gubbins ⁴ , Siddharth Jayaraman ⁵ , Basudev Paudyal ⁴ , Sophie Morgan ⁴ , Adam McNee ⁴ , Théo Morin ⁶ , Pramila Rijal ⁷ , Wilhelm Gerner ⁴ , Andrew K Sewell ⁶ , Ryo Inoue ⁸ , Mick Bailey ⁹ , Timothy Connelley ⁵ , Bryan Charleston ⁴ , Alain Townsend ⁷ , Peter Beverley ¹⁰ , Elma Tchilian ¹¹



Affiliations

• PMID: 35145208
• DOI: 10.1038/s41385-021-00478-4

Abstract

For the first time we have defined naïve, central memory, effector memory and differentiated effector porcine CD8 T cells and analyzed their distribution in lymphoid and respiratory tissues after influenza infection or immunization, using peptide-MHC tetramers of three influenza nucleoprotein (NP) epitopes. The hierarchy of response to the three epitopes changes during the response in different tissues. Most NP-specific CD8 T cells in broncho-alveolar lavage (BAL) and lung are tissue resident memory cells (TRM) that express CD69 and downregulate CD45RA and CCR7. NP-specific cells isolated from BAL express genes characteristic of TRM, but gene expression differs at 7, 21 and 63 days post infection. In all tissues the frequency of NP-specific CD8 cells declines over 63 days almost to background levels but is best maintained in BAL. The kinetic of influenza specific memory CD8 T cell in this natural host species differs from that in small animal models.