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PLoS. Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

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  • PLoS. Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

    Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk (PLoS, abstract, edited)


    [Source: PLoS, full text: <cite cite="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012591">PLoS ONE: Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk</cite>. Abstract, edited.]

    Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk


    Yanlong Cong 1#, Guangmei Wang 1#, Zhenhong Guan 2#, Shuang Chang 1, Quanpeng Zhang 1, Guilian Yang 3, Weili Wang 4, Qingfeng Meng 4, Weiming Ren 3, Chunfeng Wang 3*, Zhuang Ding 1*
    1 College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, China,
    2 Institute of Zoonoses, Jilin University, Changchun, Jilin, China,
    3 College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China,
    4 Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, Jilin, China


    Abstract

    Background
    Human-like H3N2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. The fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. However, the determination of whether pigs can act as a “mixing vessel” for a possible future pandemic virus is still pending an answer. This prompted us to gather the epidemiological information and investigate the genetic evolution of swine influenza viruses in Jilin, China.

    Methods
    Nasopharyngeal swabs were collected from pigs with respiratory illness in Jilin province, China from July 2007 to October 2008. All samples were screened for influenza A viruses. Three H3N2 swine influenza virus isolates were analyzed genetically and phylogenetically.

    Results
    Influenza surveillance of pigs in Jilin province, China revealed that H3N2 influenza viruses were regularly detected from domestic pigs during 2007 to 2008. Phylogenetic analysis revealed that two distinguishable groups of H3N2 influenza viruses were present in pigs: the wholly contemporary human-like H3N2 viruses (represented by the Moscow/10/99-like sublineage) and double-reassortant viruses containing genes from contemporary human H3N2 viruses and avian H5 viruses, both co-circulating in pig populations.

    Conclusions
    The present study reports for the first time the coexistence of wholly human-like H3N2 viruses and double-reassortant viruses that have emerged in pigs in Jilin, China. It provides updated information on the role of pigs in interspecies transmission and genetic reassortment of influenza viruses.

    Citation: Cong Y, Wang G, Guan Z, Chang S, Zhang Q, et al. (2010) Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk. PLoS ONE 5(9): e12591. doi:10.1371/journal.pone.0012591

    Editor: Ramy K. Aziz, Cairo University, Egypt
    Received: January 29, 2010; Accepted: July 26, 2010; Published: September 7, 2010
    Copyright: © 2010 Cong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

    Funding: This study was supported by the Jilin University Scientific Research Startup Fund (4305050102B6), the Jilin University Basic Science Research Fund (200903334) and the National Natural Scientific Fund (30972192). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

    Competing interests: The authors have declared that no competing interests exist.

    * E-mail: congyanlong@126.com (ZD); wangchunfeng@jlau.edu.cn (CW)
    # These authors contributed equally to this work.

    -
    ------

  • #2
    Re: PLoS. Reassortant between H3N2(1,..,6) and LP-H5(7,8) in Pigs

    http://www.ncbi.nlm.nih.gov/pmc/arti...9/?tool=pubmed

    H3N2 in Jilin pigs in 2007-2008
    {2-3 years ago ! if it were important, they had told us earlier ?!?!}
    {OK, sequences were uploaded 2009/11/30 so only 1-2 years}


    1.) Moscow/10/99-like
    2.) "double-reassortant : contemporary human H3N2 + avian H5
    {HP ?}


    double-reassortant viruses containing genes from
    contemporary human (PB2, PB1, PA, HA, NP, and NA)
    and avian H5 (M and NS)

    Sw/Jilin/37/08
    Sw/Jilin/5/07
    Sw/Jilin/19/07

    H5N3 viruses (A/Dk/Altai/1285/1991,
    A/Dk/Primorie/2633/2001,
    A/Anasplatyrhynchos/Altai/1285/1991,
    A/Migratory Dk/Jiangxi/13487/2005
    The NS gene of Sw/Jilin/37/08 had a close relationship with
    A/Mallard/Italy/4223-2/2006 (H5N2) and
    A/Dk/Primorie/2633/2001 (H5N3)


    ahh, LP H5N3 and H5N2
    not the 15BP deletion in NS=segment 8


    total of 279 nasopharyngeal swabs were collected in Jilin province,
    China from July 2007 to October 2008


    GU215015 - GU215038

    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215015
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215016
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215017
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215018
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215019
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215020
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215021
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215022

    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215023
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215024
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215025
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215026
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215027
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215028
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215029
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215030

    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215031
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215032
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215033
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215034
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215035
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215036
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215037
    http://www.ncbi.nlm.nih.gov/entrez/v...i?val=GU215038



    Submitted (18-NOV-2009) Laboratory of Infectious Diseases, College
    of Animal Science and Veterinary Medicine, Jilin University, 5333
    Xi'an Road, Changchun, Jilin 130062, China

    not the Harbin lab
    I'm interested in expert panflu damage estimates
    my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

    Comment


    • #3
      Re: PLoS. Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

      just only the one 2008-virus has reassorted
      the H3 went from human to swine ~10 years ago

      differences in promille of available nucleotides in the coding region
      Code:
        1 >A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844   
        1:  0,  0,  0,  0,  0,  0,  0,  0   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2:  0, 10,  7, 13, 56,  4,  2,  4   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3:  6,  9,  4, 24,  2, 33,114,133   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4: 23, 28, 29, 42, 28, 40, 12, 15   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5: 10,  9, 12, 15, 18, 21,  8,  9   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6: 15, 15, 15, 34, 15, 18, 15,  9   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:147, 36,171, 49,166, 24,111,171   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        2 >A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844   
        1:  0, 10,  7, 13, 56,  4,  2,  4   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2:  0,  0,  0,  0,  0,  0,  0,  0   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3:  5,  8,  3, 14, 56, 33,112,133   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4: 24, 25, 26, 48, 63, 40, 10, 15   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5: 11,  8,  8, 25, 60, 21,  6,  9   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6: 16, 15, 12, 45, 61, 19, 13,  9   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:147, 33,168, 58,156, 24,109,171   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        3 >A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844   
        1:  6,  9,  4, 24,  2, 33,114,133   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2:  5,  8,  3, 14, 56, 33,112,133   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3:  0,  0,  0,  0,  0,  0,  0,  0   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4: 24, 25, 27, 53, 30, 58,107,130   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5: 11,  6,  9, 29, 20, 46,113,132   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6: 16, 15, 13, 51, 16, 42,113,130   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:146, 31,169, 62,168, 48,111,153   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        4 >1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844   
        1: 23, 28, 29, 42, 28, 40, 12, 15   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2: 24, 25, 26, 48, 63, 40, 10, 15   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3: 24, 25, 27, 53, 30, 58,107,130   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4:  0,  0,  0,  0,  0,  0,  0,  0   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5: 18, 21, 19, 31, 16, 29,  8,  8   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6: 31, 32, 26, 56, 29, 48, 18, 17   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:152, 25,161, 34,162, 31,106,170   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        5 >1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844   
        1: 10,  9, 12, 15, 18, 21,  8,  9   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2: 11,  8,  8, 25, 60, 21,  6,  9   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3: 11,  6,  9, 29, 20, 46,113,132   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4: 18, 21, 19, 31, 16, 29,  8,  8   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5:  0,  0,  0,  0,  0,  0,  0,  0   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6: 15, 15, 10, 28, 19, 29, 16, 11   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:156, 29,165, 39,167, 12,108,169   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        6 >2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842   
        1: 15, 15, 15, 34, 15, 18, 15,  9   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2: 16, 15, 12, 45, 61, 19, 13,  9   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3: 16, 15, 13, 51, 16, 42,113,130   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4: 31, 32, 26, 56, 29, 48, 18, 17   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5: 15, 15, 10, 28, 19, 29, 16, 11   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6:  0,  0,  0,  0,  0,  0,  0,  0   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:156, 39,168, 64,168, 31,112,169   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      
        7 >A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844   
        1:147, 36,171, 49,166, 24,111,171   A/Sw/Jilin/5/2007/09/21(H3N2)              1121 2274  909 1701 1497 1409  957  844
        2:147, 33,168, 58,156, 24,109,171   A/Sw/Jilin/19/2007/11/09(H3N2)             1121 2273  909 1700 1497 1410  956  844
        3:146, 31,169, 62,168, 48,111,153   A/Sw/Jilin/37/2008/10/30(H3N2)             1121 2273  909 1701 1496 1410  957  844
        4:152, 25,161, 34,162, 31,106,170   1989//,7852,CHN,A/Beijing/353/89           2280 2272 2151 1701 1497 1409  982  844
        5:156, 29,165, 39,167, 12,108,169   1997/11/29,10924,USA,A/NY/503/97           2280 2274 2151 1701 1497 1410  982  844
        6:156, 39,168, 64,168, 31,112,169   2003/04/23,12897,USA,A/NY/214/03           2280 2274 2151 1701 1497 1410  975  842
        7:  0,  0,  0,  0,  0,  0,  0,  0   A/Sw/Index/triple-reassortant/1998(H3N2)   2280 2274 2151 1701 1497 1410  982  844
      I'm interested in expert panflu damage estimates
      my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

      Comment


      • #4
        Re: PLoS. Reassortant between Human-Like H3N2 and Avian H5 Subtype Influenza A Viruses in Pigs: A Potential Public Health Risk

        M1:
        0 0 751 2728 >A/Sw/Jilin/37/2008/10/30(H3N2)
        146 11 751 1149 >A/Anas platyrhynchos/Altai/1285/1991(H5N3),1991,0,3,0
        146 11 751 1152 >A/Dk/Altai/1285/1991(H5N3)08/15,1991,10,3,0
        159 12 751 736 >A/migratory Dk/Jiang Xi/13487/05(H5N3),2005,10,2,0
        159 12 751 1202 >A/mallard/Netherlands/02/00(H10N7),2000,3,3,0
        173 13 751 154 >A/Dk/Taiwan/WB29/99(H6N1),1999,10,2,1

        NS:
        0 0 838 3263 >A/Sw/Jilin/37/2008/10/30(H3N2)
        143 12 836 1704 >A/Dk/Primorie/2633/01(H5N3),2001,10,3,0
        143 12 838 427 >A/Dk/MNG/47/01(H7N1),2001,10,2,0
        143 12 838 1708 >A/Dk/Primorie/2633/01(H5N3)08/24,2001,10,3,0
        143 12 838 1899 >A/mallard/Italy/4223-2/06(H5N2)11/11,2006,3,3,0
        155 13 838 496 >A/Dk/Yunnan/435/02(H5N3),2002,10,2,0
        155 13 838 690 >A/Dk/Jiangxi/1742/03(H7N7),2003,10,2,0
        155 13 838 1728 >A/Anas querquedula/Astrakhan/3091/02(H4N8)09/09,2002,0,3,0
        155 13 838 1733 >A/mallard/Denmark/64650/03(H5N7),2003,3,3,0
        168 14 830 1233 >A/aquatic bird/Korea/KN-4/05(H3N8),2005,0,2,0
        168 14 831 6 >A/teal/Egypt/912908/05(H10N7),2005,8,1,0


        5 differences to the bird-index in segment 7
        1 difference to the bird-index in segment 8

        how do pigs live in Jilin,do they have contact with ducks ?
        I'm interested in expert panflu damage estimates
        my current links: [url]http://bit.ly/hFI7H[/url] ILI-charts: [url]http://bit.ly/CcRgT[/url]

        Comment

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