J Vet Diagn Invest . Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

[tetano]

J Vet Diagn Invest


. 2021 Feb 22;1040638721994810.
doi: 10.1177/1040638721994810. Online ahead of print.
Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses


Yixin Xiao ¹ , Fan Yang ¹ , Fumin Liu ¹ , Hangping Yao ¹ , Nanping Wu ¹ , Haibo Wu ¹



Affiliations

• PMID: 33618630
• DOI: 10.1177/1040638721994810

Abstract

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID₅₀)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

Keywords: H2 subtype; avian influenza A virus; real-time RT-PCR.