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Genetic and pathogenic characteristics of clade 2.3.2.1c H5N1 highly pathogenic avian influenza viruses isolated from poultry outbreaks in Laos during 2015-2018

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  • Genetic and pathogenic characteristics of clade 2.3.2.1c H5N1 highly pathogenic avian influenza viruses isolated from poultry outbreaks in Laos during 2015-2018


    Transbound Emerg Dis. 2019 Nov 26. doi: 10.1111/tbed.13430. [Epub ahead of print] Genetic and pathogenic characteristics of clade 2.3.2.1c H5N1 highly pathogenic avian influenza viruses isolated from poultry outbreaks in Laos during 2015-2018.

    Park YR1,2, Lee YN1, Lee DH3, Baek YG1, Si YJ1, Meeduangchanh P4, Theppangna W4, Douangngeun B4, Kye SJ1, Lee MH1, Park CK2, Lee YJ1.
    Author information

    1 Avian Influenza Research & Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon, Republic of Korea. 2 College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University, Daegu, Republic of Korea. 3 Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT, USA. 4 National Animal Health Laboratory, Vientiane, Lao PDR.

    Abstract

    Since 2004, there have been multiple outbreaks of H5 highly pathogenic avian influenza (HPAI) viruses in Laos. Here, we isolated H5N1 HPAI viruses from poultry outbreaks in Laos during 2015-2018 and investigated their genetic characteristics and pathogenicity in chickens. Phylogenetic analysis revealed that the isolates belonged to clade 2.3.2.1c, and that they differed from previous Laos viruses with respect to genetic composition. In particular, the isolates were divided into two genotypes, each of which had a different NS segment. The results of possible migration analysis suggested a high likelihood that the Laos isolates were introduced from neighboring countries, particularly Vietnam. The recent Laos isolate, A/Duck/Laos/NL-1504599/2018, had an intravenous pathogenicity index score of 3.0 and showed a 50% chicken lethal dose of 102.5 EID50 /0.1 ml, indicating high pathogenicity. The isolated viruses exhibited no critical substitution in the markers associated with mammalian adaptation, but possess markers related to neuraminidase inhibitor resistance. These results emphasize the need for ongoing surveillance of circulating influenza virus in Southeast Asia, including Laos, to better prepare for and mitigate global spread of H5 HPAI.
    2019 Blackwell Verlag GmbH.


    KEYWORDS:

    H5N1; Influenza A virus; Laos; Phylogenetic analysis

    PMID: 31769586 DOI: 10.1111/tbed.13430

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