Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challenge

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Vaccine. 2019 Nov 14. pii: S0264-410X(19)31513-0. doi: 10.1016/j.vaccine.2019.11.006. [Epub ahead of print] Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challenge.

Sister?-Or? M¹, Mart?nez-Pulgar?n S², Solanes D³, Veljkovic V⁴, L?pez-Serrano S⁵, C?rdoba L⁶, Cord?n I⁷, Escribano JM², Darji A⁸.
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1 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. 2 Alternative Gene Expression S.L. ALGENEX, Centro empresarial - Parque Cient?fico y Tecnol?gico Universidad Polit?cnica de Madrid Campus de Montegancedo, 28223 Pozuelo de Alarc?n, Spain. 3 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: david.solanes@irta.cat. 4 Biomed Protection, Galveston, 77550 TX, USA. 5 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: sergi.lopez@irta.cat. 6 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: lorena.cordoba@irta.cat. 7 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ivan.cordon@irta.cat. 8 IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Aut?noma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ayub.darji@irta.cat.

Abstract

The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing "flagellin-NG34/CS17 antigen" were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CS17 vaccine protected the vaccinated chickens and blocked viral shedding orally and cloacally. Furthermore, no apparent clinical signs were monitored in 10/12 vaccinated individuals. The mechanism of protection conferred is under investigation.
Copyright ? 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.


KEYWORDS:

Avian influenza; Baculovirus; Conserved peptides; Cross-protection; Flagellin; H7 subtype; Influenza vaccine; Pandemic flu

PMID: 31735501 DOI: 10.1016/j.vaccine.2019.11.006
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