Reverse transcription recombinase-aided amplification assay for H7 subtype avian influenza virus

[tetano]

Transbound Emerg Dis. 2019 Nov 12. doi: 10.1111/tbed.13411. [Epub ahead of print] Reverse transcription recombinase-aided amplification assay for H7 subtype avian influenza virus.

Wang S¹, Huang B¹, Ma X², Liu P¹, Wang Y³, Zhang X², Zhu L¹, Fan Q³, Sun Y³, Wang K¹.
Author information

1 China Animal Health and Epidemiology Center, Qingdao, Shandong Province, China. 2 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. 3 College of Animal Science and Technology, Henan University of Science and Technology, Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang.

Abstract

H7 subtype avian influenza virus infection is an emerging zoonosis in some Asian countries and an important avian disease worldwide. A rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. In this study, we developed a reverse transcription recombinase-aided amplification assay for the detection of H7 subtype avian influenza virus. Assays were performed at a single temperature (39?C) and the results were obtained within 20 min. The assay showed no cross-detection with Newcastle disease virus or infectious bronchitis virus, which are the other main respiratory viruses affecting birds. The analytical sensitivity was 10² RNA copies per reaction at a 95% probability level according to probit regression analysis, with 100% specificity. Compared with published reverse transcription quantitative real-time polymerase chain reaction assays, the κ value of the reverse transcription recombinase-aided amplification assay in 342 avian clinical samples was 0.988 (p < .001). The sensitivity for avian clinical sample detection was 100% (95%CI, 90.40%-100%) and the specificity was 99.96% (95%CI, 97.83%-99.98%). These results indicated that our reverse transcription recombinase-aided amplification assay may be a valuable tool for detecting avian influenza H7 subtype virus.
? 2019 Blackwell Verlag GmbH.


KEYWORDS:

H7 subtype; avian influenza; reverse transcription recombinase-aided amplification assay

PMID: 31714018 DOI: 10.1111/tbed.13411