Tweet
Sheng Wu Gong Cheng Xue Bao. 2019 Jun 25;35(6):1029-1040. doi: 10.13345/j.cjb.180490.
[Inhibition of the replication of H9N2 influenza virus in vivo by short-term repeated oral administration of chicken interferon α].
[Article in Chinese; Abstract available in Chinese from the publisher]
Wang M1,2, Song J1,2, Fan W1, Liu L1,2, Huang Z3, Yang C3, Wu H3, Liu W1,2, Li J1,2.
Author information
Abstract
in English, Chinese
To evaluate the optimal administration frequency for interferon-α (IFN-α) and the effect of its combined use with inactive virus on chicken flocks, the prokaryotic expression plasmid pET-22b-ChIFN-α was constructed and transferred into Escherichia coli BL21(DE3) host bacteria to induce the expression of chicken IFN-α and to harvest recombinant proteins inclusion bodies. The expression of recombinant chicken IFN-α was confirmed by SDS-PAGE, and the results demonstrated that the chicken IFN-α (20 kDa) was highly expressed using the prokaryotic expression vector with a concentration of 0.2 mg/mL in the medium. Chicken IFN-α was diluted to 2.5?10⁴ U/fowls and administered to immunized specific-pathogen-free chickens orally in combination with inactivated H9N2 subtype influenza virus. Chicken that received chicken IFN-α were safe after three repeated immunizations (96 h). In addition, chicken IFN-α could induce higher levels of antiviral-related inducible genes in peripheral blood, spleen, and thymus of chicken flocks. The results of a challenge assay revealed that the lowest detoxification rates of chicken IFN-α ranged from three to five days, suggesting a higher capacity to resist H9N2 subtype avian influenza virus. The present study obtained the optimal immune frequency and immunization period for chicken IFN-α to provide theoretical support for the optimal clinical application of IFN-α.
KEYWORDS:
H9N2 influenza viruses; chicken interferon α; oral administration; short-term repeated
PMID: 31231999 DOI: 10.13345/j.cjb.180490
[Inhibition of the replication of H9N2 influenza virus in vivo by short-term repeated oral administration of chicken interferon α].
[Article in Chinese; Abstract available in Chinese from the publisher]
Wang M1,2, Song J1,2, Fan W1, Liu L1,2, Huang Z3, Yang C3, Wu H3, Liu W1,2, Li J1,2.
Author information
Abstract
in English, Chinese
To evaluate the optimal administration frequency for interferon-α (IFN-α) and the effect of its combined use with inactive virus on chicken flocks, the prokaryotic expression plasmid pET-22b-ChIFN-α was constructed and transferred into Escherichia coli BL21(DE3) host bacteria to induce the expression of chicken IFN-α and to harvest recombinant proteins inclusion bodies. The expression of recombinant chicken IFN-α was confirmed by SDS-PAGE, and the results demonstrated that the chicken IFN-α (20 kDa) was highly expressed using the prokaryotic expression vector with a concentration of 0.2 mg/mL in the medium. Chicken IFN-α was diluted to 2.5?10⁴ U/fowls and administered to immunized specific-pathogen-free chickens orally in combination with inactivated H9N2 subtype influenza virus. Chicken that received chicken IFN-α were safe after three repeated immunizations (96 h). In addition, chicken IFN-α could induce higher levels of antiviral-related inducible genes in peripheral blood, spleen, and thymus of chicken flocks. The results of a challenge assay revealed that the lowest detoxification rates of chicken IFN-α ranged from three to five days, suggesting a higher capacity to resist H9N2 subtype avian influenza virus. The present study obtained the optimal immune frequency and immunization period for chicken IFN-α to provide theoretical support for the optimal clinical application of IFN-α.
KEYWORDS:
H9N2 influenza viruses; chicken interferon α; oral administration; short-term repeated
PMID: 31231999 DOI: 10.13345/j.cjb.180490