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Eur J Pharm Biopharm. 2018 Oct 9. pii: S0939-6411(18)30806-3. doi: 10.1016/j.ejpb.2018.10.008. [Epub ahead of print]
Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge.
Tomar J1, Biel C1, de Haan CAM2, Rottier PJM2, Petrovsky N3, Frijlink HW1, Huckriede A4, Hinrichs WLJ1, Peeters B5.
Author information
Abstract
Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.
KEYWORDS:
Passive; adjuvants; challenge; influenza; inhalation; powders; protection; pulmonary
PMID: 30312742 DOI: 10.1016/j.ejpb.2018.10.008
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Passive inhalation of dry powder influenza vaccine formulations completely protects chickens against H5N1 lethal viral challenge.
Tomar J1, Biel C1, de Haan CAM2, Rottier PJM2, Petrovsky N3, Frijlink HW1, Huckriede A4, Hinrichs WLJ1, Peeters B5.
Author information
Abstract
Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge. The aim of the present study was to improve the effectiveness of pulmonary vaccination by increasing the vaccine dose deposited in the lungs and by the use of suitable adjuvants. Two adjuvants, namely, Bacterium-like Particles (BLP) and Advax, were spray freeze dried with influenza vaccine into dry powder formulations. Delivery of dry formulations directly at the syrinx revealed that BLP and Advax had the potential to boost either systemic or mucosal immune responses or both. Upon passive inhalation of dry influenza vaccine formulations in an optimized set-up, BLP and Advax/BLP adjuvanted formulations induced significantly higher systemic immune responses than the non-adjuvanted formulation. Remarkably, all vaccinated animals not only survived a lethal influenza challenge, but also did not show any shedding of challenge virus except for two out of six animals in the Advax group. Overall, our results indicate that passive inhalation is feasible, effective and suitable for mass vaccination of chickens if it can be adapted to field settings.
KEYWORDS:
Passive; adjuvants; challenge; influenza; inhalation; powders; protection; pulmonary
PMID: 30312742 DOI: 10.1016/j.ejpb.2018.10.008
Free full text