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VOC breath profile in spontaneously breathing awake swine during Influenza A infection

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  • VOC breath profile in spontaneously breathing awake swine during Influenza A infection

    Sci Rep. 2018 Oct 5;8(1):14857. doi: 10.1038/s41598-018-33061-2.
    VOC breath profile in spontaneously breathing awake swine during Influenza A infection.

    Traxler S1, Bischoff AC1, Sa▀ R1, Trefz P1, Gierschner P1, Brock B1, Schwaiger T2, Karte C3, Blohm U4, Schr÷der C2, Miekisch W5, Schubert JK1.
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    Abstract

    Influenza is one of the most common causes of virus diseases worldwide. Virus detection requires determination of Influenza RNA in the upper respiratory tract. Efficient screening is not possible in this way. Analysis of volatile organic compounds (VOCs) in breath holds promise for non-invasive and fast monitoring of disease progression. Breath VOC profiles of 14 (3 controls and 11 infected animals) swine were repeatedly analyzed during a complete infection cycle of Influenza A under high safety conditions. Breath VOCs were pre-concentrated by means of needle trap micro-extraction and analysed by gas chromatography mass spectrometry before infection, during virus presence in the nasal cavity, and after recovery. Six VOCs could be related to disease progression: acetaldehyde, propanal, n-propyl acetate, methyl methacrylate, styrene and 1,1-dipropoxypropane. As early as on day four after inoculation, when animals were tested positive for Influenza A, differentiation between control and infected animals was possible. VOC based information on virus infection could enable early detection of Influenza A. As VOC analysis is completely non-invasive it has potential for large scale screening purposes. In a perspective, breath analysis may offer a novel tool for Influenza monitoring in human medicine, animal health control or border protection.


    PMID: 30291257 DOI: 10.1038/s41598-018-33061-2
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