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Sci Rep. 2018 Mar 19;8(1):4801. doi: 10.1038/s41598-018-23022-0.
Experimental infection of Cynomolgus Macaques with highly pathogenic H5N1 influenza virus through the aerosol route.
Watanabe T1, Iwatsuki-Horimoto K2, Kiso M2, Nakajima N3, Takahashi K3, Jose da Silva Lopes T4, Ito M2, Fukuyama S2, Hasegawa H3, Kawaoka Y5,6,7.
Author information
Abstract
Several animal models are used to study influenza viruses. Intranasal inoculation of animals with a liquid inoculum is one of the main methods used to experimentally infect animals with influenza virus; however, this method does not reflect the natural infection with influenza virus by contact or aerosol route. Aerosol inhalation methods have been established with several influenza viruses for mouse and ferret models, but few studies have evaluated inoculation routes in a nonhuman primates (NHP) model. Here, we performed the experimental infection of NHPs with a highly pathogenic H5N1 influenza virus via the aerosol route and demonstrated that aerosol infection had no effect on clinical outcome, but caused broader infection throughout all of the lobes of the lung compared with a non-aerosolized approach. Aerosol infection therefore represents an option for inoculation of NHPs in future studies.
PMID: 29556081 PMCID: PMC5859186 DOI: 10.1038/s41598-018-23022-0
Free PMC Article
Experimental infection of Cynomolgus Macaques with highly pathogenic H5N1 influenza virus through the aerosol route.
Watanabe T1, Iwatsuki-Horimoto K2, Kiso M2, Nakajima N3, Takahashi K3, Jose da Silva Lopes T4, Ito M2, Fukuyama S2, Hasegawa H3, Kawaoka Y5,6,7.
Author information
Abstract
Several animal models are used to study influenza viruses. Intranasal inoculation of animals with a liquid inoculum is one of the main methods used to experimentally infect animals with influenza virus; however, this method does not reflect the natural infection with influenza virus by contact or aerosol route. Aerosol inhalation methods have been established with several influenza viruses for mouse and ferret models, but few studies have evaluated inoculation routes in a nonhuman primates (NHP) model. Here, we performed the experimental infection of NHPs with a highly pathogenic H5N1 influenza virus via the aerosol route and demonstrated that aerosol infection had no effect on clinical outcome, but caused broader infection throughout all of the lobes of the lung compared with a non-aerosolized approach. Aerosol infection therefore represents an option for inoculation of NHPs in future studies.
PMID: 29556081 PMCID: PMC5859186 DOI: 10.1038/s41598-018-23022-0
Free PMC Article