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Arch Virol. 2018 Feb 21. doi: 10.1007/s00705-018-3773-2. [Epub ahead of print]
Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses.
Yang F1, Wu H2, Liu F1, Lu X1, Peng X1, Wu N3.
Author information
Abstract
The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.
KEYWORDS:
Avian influenza virus; Detection; H6 subtype; Rapid; Sensitivity
PMID: 29468361 DOI: 10.1007/s00705-018-3773-2
Establishment of a multiplex real-time RT-PCR assay for rapid identification of H6 subtype avian influenza viruses.
Yang F1, Wu H2, Liu F1, Lu X1, Peng X1, Wu N3.
Author information
Abstract
The H6 subtype avian influenza viruses (AIVs) possess the capacity for zoonotic transmission from avian species to humans. Establishment of a specific, rapid and sensitive method to screen H6 AIVs is necessary. Based on the conserved domain of the matrix and H6 AIV hemagglutinin genes, two TaqMan minor-groove-binder probes and multiplex real-time RT-PCR primers were designed in this study. The multiplex real-time RT-PCR assay developed in this study had high specificity and repeatability and a detection limit of 30 copies per reaction. This rapid diagnostic method will be useful for clinical detection and surveillance of H6 AIVs in China.
KEYWORDS:
Avian influenza virus; Detection; H6 subtype; Rapid; Sensitivity
PMID: 29468361 DOI: 10.1007/s00705-018-3773-2