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Avian Pathol. 2018 Jan 19:1-28. doi: 10.1080/03079457.2018.1430891. [Epub ahead of print]
Disclosing respiratory coinfections: a Broad-Range Panel Assay for Avian Respiratory Pathogens on a Nanofluidic PCR Platform.
Croville G1, Foret C1, Heuillard P1, Senet A1, Delpont M1, Mouahid M2, Ducatez MF1, Kichou F3, Guerin JL1.
Author information
Abstract
Respiratory syndromes (RS) are among the most significant pathological conditions in food animals and are caused by complex coactions of pathogens and environmental factors. In poultry, low pathogenic avian Influenza A viruses, metapneumoviruses, infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma spp. Escherichia coli and/or Ornithobacterium rhinotracheale (ORT) in turkeys, are considered as key co-infectious agents of respiratory syndromes. Aspergillus sp., Pasteurella multocida, Avibacterium paragallinarum or Chlamydia psittaci may also be involved in respiratory outbreaks. An innovative quantitative PCR method, based on a nanofluidic technology, has the ability to screen up to 96 samples with 96 pathogen-specific PCR primers, at the same time, in one run of real-time quantitative PCR (RTqPCR). This platform was used for the screening of avian respiratory pathogens: 15 respiratory agents, including viruses, bacteria and fungi potentially associated with respiratory infections of poultry were targeted. Primers were designed and validated for SYBR green RTqPCR and subsequently validated on the Biomark high throughput PCR nanofluidic platform (Fluidigm?). As a clinical assessment, tracheal swabs were sampled on turkeys showing respiratory syndromes and submitted to this panel assay. Beside systematic detection of E. coli, avian metapneumovirus, Mycoplasma gallisepticum and M. synoviae were frequently detected, with distinctive co-infection patterns between French and Moroccan flocks. This proof-of-concept study illustrates the potential of such panel assay for unveiling respiratory co-infections profiles in poultry.
KEYWORDS:
Avian respiratory pathogens; High throughput PCR; coinfections; microfluidic PCR
PMID: 29350071 DOI: 10.1080/03079457.2018.1430891
Disclosing respiratory coinfections: a Broad-Range Panel Assay for Avian Respiratory Pathogens on a Nanofluidic PCR Platform.
Croville G1, Foret C1, Heuillard P1, Senet A1, Delpont M1, Mouahid M2, Ducatez MF1, Kichou F3, Guerin JL1.
Author information
Abstract
Respiratory syndromes (RS) are among the most significant pathological conditions in food animals and are caused by complex coactions of pathogens and environmental factors. In poultry, low pathogenic avian Influenza A viruses, metapneumoviruses, infectious bronchitis virus, infectious laryngotracheitis virus, Mycoplasma spp. Escherichia coli and/or Ornithobacterium rhinotracheale (ORT) in turkeys, are considered as key co-infectious agents of respiratory syndromes. Aspergillus sp., Pasteurella multocida, Avibacterium paragallinarum or Chlamydia psittaci may also be involved in respiratory outbreaks. An innovative quantitative PCR method, based on a nanofluidic technology, has the ability to screen up to 96 samples with 96 pathogen-specific PCR primers, at the same time, in one run of real-time quantitative PCR (RTqPCR). This platform was used for the screening of avian respiratory pathogens: 15 respiratory agents, including viruses, bacteria and fungi potentially associated with respiratory infections of poultry were targeted. Primers were designed and validated for SYBR green RTqPCR and subsequently validated on the Biomark high throughput PCR nanofluidic platform (Fluidigm?). As a clinical assessment, tracheal swabs were sampled on turkeys showing respiratory syndromes and submitted to this panel assay. Beside systematic detection of E. coli, avian metapneumovirus, Mycoplasma gallisepticum and M. synoviae were frequently detected, with distinctive co-infection patterns between French and Moroccan flocks. This proof-of-concept study illustrates the potential of such panel assay for unveiling respiratory co-infections profiles in poultry.
KEYWORDS:
Avian respiratory pathogens; High throughput PCR; coinfections; microfluidic PCR
PMID: 29350071 DOI: 10.1080/03079457.2018.1430891