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Infect Genet Evol. 2017 Aug 10. pii: S1567-1348(17)30262-9. doi: 10.1016/j.meegid.2017.08.005. [Epub ahead of print]
Molecular detection and characterization of Influenza 'C' viruses from western India.
Potdar VA1, Hinge DD2, Dakhave MR2, Manchanda A2, Jadhav N2, Kulkarni PB2, Chadha MS2.
Author information
Abstract
Since 2003, India has had a well-established influenza surveillance network, though Influenza C virus was not the focus of study. We therefore retrospectively analyzed clinical samples from Pune, western India collected during January 2009 to August 2015, by real-time RT-PCR. Three of 2530 samples of patients with influenza-like illness (ILI) or severe acute respiratory illness (SARI) showed positivity for Influenza C virus infection, while 105 and 31 samples were positive for Influenza A and B viruses respectively. Influenza C viruses were successfully isolated using the embryonated egg system and whole genomes were sequenced and analyzed phylogenetically. HE gene-based phylogeny showed that two viruses C/India/P119564/2011 and C/India P121719/2012 clustered with the C/Sao Paulo/378/82 (SP82) lineage, whereas C/India/P135047/2013 clustered with the C/Kanagawa/1/76 (KA76) lineage. The internal gene of these viruses grouped in two lineages. The PB1, PB2, M and NS genes of the study viruses grouped with C/Yamagata/26/81 (YA81), while the P3 (PA) and NP genes grouped with C/Mississippi/80 (MS80). Bayesian clock studies conclude that the Indian strains may have emerged through multiple reassortment events.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
IPD/OPD; Influenza C virus; Real time PCR; Western India
PMID: 28803969 DOI: 10.1016/j.meegid.2017.08.005
Molecular detection and characterization of Influenza 'C' viruses from western India.
Potdar VA1, Hinge DD2, Dakhave MR2, Manchanda A2, Jadhav N2, Kulkarni PB2, Chadha MS2.
Author information
Abstract
Since 2003, India has had a well-established influenza surveillance network, though Influenza C virus was not the focus of study. We therefore retrospectively analyzed clinical samples from Pune, western India collected during January 2009 to August 2015, by real-time RT-PCR. Three of 2530 samples of patients with influenza-like illness (ILI) or severe acute respiratory illness (SARI) showed positivity for Influenza C virus infection, while 105 and 31 samples were positive for Influenza A and B viruses respectively. Influenza C viruses were successfully isolated using the embryonated egg system and whole genomes were sequenced and analyzed phylogenetically. HE gene-based phylogeny showed that two viruses C/India/P119564/2011 and C/India P121719/2012 clustered with the C/Sao Paulo/378/82 (SP82) lineage, whereas C/India/P135047/2013 clustered with the C/Kanagawa/1/76 (KA76) lineage. The internal gene of these viruses grouped in two lineages. The PB1, PB2, M and NS genes of the study viruses grouped with C/Yamagata/26/81 (YA81), while the P3 (PA) and NP genes grouped with C/Mississippi/80 (MS80). Bayesian clock studies conclude that the Indian strains may have emerged through multiple reassortment events.
Copyright ? 2017. Published by Elsevier B.V.
KEYWORDS:
IPD/OPD; Influenza C virus; Real time PCR; Western India
PMID: 28803969 DOI: 10.1016/j.meegid.2017.08.005