New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses

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J Virol Methods. 2017 Mar 20. pii: S0166-0934(16)30210-5. doi: 10.1016/j.jviromet.2017.02.018. [Epub ahead of print]
New real time and conventional RT-PCRs for updated molecular diagnosis of infectious bronchitis virus infection (IBV) in chickens in Egypt associated with frequent co-infections with avian influenza and Newcastle Disease viruses.

Naguib MM¹, El-Kady MF², L?schow D³, Hassan KE², Arafa AS⁴, El-Zanaty A⁴, Hassan MK⁴, Hafez HM³, Grund C⁵, Harder TC⁶.
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Abstract

In Egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (IBV) are circulating with detrimental effects for poultry industry. A sensitive real-time RT-PCR assay targeting the IBV nucleocapsid gene (N) was developed to screen clinical samples for presence of IBV. Conventional RT-PCRs amplifying hypervariable regions (HVRs 1-2 and 3) of the IBV S1 gene were developed and amplificates used for nucleotide sequence-based typing of IBV field strains in Egyptian chickens directly from clinical samples.
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PMID: 28336367 DOI: 10.1016/j.jviromet.2017.02.018