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Vet Res. 2017 Feb 6;48(1):7. doi: 10.1186/s13567-017-0410-0.
The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs.
Gu M1,2, Li Q1, Gao R1, He D1, Xu Y1, Xu H1, Xu L1,3, Wang X1,2, Hu J1,2, Liu X1,2, Hu S1,2, Peng D1,2,4, Jiao X1,2,4, Liu X5,6,7.
Author information
Abstract
We generated and characterized site-directed HA mutants on the genetic backbone of H5N1 clade 2.3.4 virus preferentially binding to α-2,3 receptors in order to identify the key determinants in hemagglutinin rendering the dual affinity to both α-2,3 (avian-type) and α-2,6 (human-type) linked sialic acid receptors of the current clade 2.3.4.4 H5NX subtype avian influenza reassortants. The results show that the T160A substitution resulted in the loss of a glycosylation site at 158N and led not only to enhanced binding specificity for human-type receptors but also transmissibility among guinea pigs, which could be considered as an important molecular marker for assessing pandemic potential of H5 subtype avian influenza isolates.
PMID: 28166830 PMCID: PMC5294818 DOI: 10.1186/s13567-017-0410-0
[PubMed - in process] Free full text
The T160A hemagglutinin substitution affects not only receptor binding property but also transmissibility of H5N1 clade 2.3.4 avian influenza virus in guinea pigs.
Gu M1,2, Li Q1, Gao R1, He D1, Xu Y1, Xu H1, Xu L1,3, Wang X1,2, Hu J1,2, Liu X1,2, Hu S1,2, Peng D1,2,4, Jiao X1,2,4, Liu X5,6,7.
Author information
Abstract
We generated and characterized site-directed HA mutants on the genetic backbone of H5N1 clade 2.3.4 virus preferentially binding to α-2,3 receptors in order to identify the key determinants in hemagglutinin rendering the dual affinity to both α-2,3 (avian-type) and α-2,6 (human-type) linked sialic acid receptors of the current clade 2.3.4.4 H5NX subtype avian influenza reassortants. The results show that the T160A substitution resulted in the loss of a glycosylation site at 158N and led not only to enhanced binding specificity for human-type receptors but also transmissibility among guinea pigs, which could be considered as an important molecular marker for assessing pandemic potential of H5 subtype avian influenza isolates.
PMID: 28166830 PMCID: PMC5294818 DOI: 10.1186/s13567-017-0410-0
[PubMed - in process] Free full text