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Avian Dis. 2016 May;60(1 Suppl):341-5. doi: 10.1637/11103-042115-Reg.
Antibodies to Influenza A Viruses in Gulls at Delaware Bay, USA.
Guinn K1, Fojtik A1, Davis-Fields N1, Poulson RL1, Krauss S2, Webster RG2, Stallknecht DE1.
Author information
Abstract
Gulls are the known reservoir for H13 and H16 influenza A viruses (IAV) but also host a diversity of other IAV subtypes. Gulls also share habitats with both ducks and shorebirds, increasing the potential for cross-species IAV transmission. We serologically tested laughing gulls (Leucophaeus atricilla) collected at Delaware Bay during May when they were in direct contact with IAV-infected shorebirds; both species feed on horseshoe crab (Limulus polyphemus) eggs on beaches during this month. From 2010 to 2014, antibody prevalence as determined by competitive blocking enzyme-linked immunosorbent assay ranged from 25%-72%. Antibodies to H13 and H16 were detected by hemagglutination inhibition (HI) tests in 12% and 24% of tested gulls, respectively. Results from virus microneutralization (MN) tests for antibodies to H1-H12, H14, and H15 varied among years but the highest prevalence of neutralizing antibodies was detected against H1 (24%), H5 (25%), H6 (35%), H9 (33%), and H11 (42%) IAV. The subtype diversity identified by serology in gulls was dominated by Group 1 HA subtypes and only partially reflected the diversity of IAV subtypes isolated from shorebirds.
KEYWORDS:
Leucophaeus atricilla microneutralization; antibodies; influenza A virus; laughing gull; serology
PMID: 27309077 [PubMed - in process]
Antibodies to Influenza A Viruses in Gulls at Delaware Bay, USA.
Guinn K1, Fojtik A1, Davis-Fields N1, Poulson RL1, Krauss S2, Webster RG2, Stallknecht DE1.
Author information
Abstract
Gulls are the known reservoir for H13 and H16 influenza A viruses (IAV) but also host a diversity of other IAV subtypes. Gulls also share habitats with both ducks and shorebirds, increasing the potential for cross-species IAV transmission. We serologically tested laughing gulls (Leucophaeus atricilla) collected at Delaware Bay during May when they were in direct contact with IAV-infected shorebirds; both species feed on horseshoe crab (Limulus polyphemus) eggs on beaches during this month. From 2010 to 2014, antibody prevalence as determined by competitive blocking enzyme-linked immunosorbent assay ranged from 25%-72%. Antibodies to H13 and H16 were detected by hemagglutination inhibition (HI) tests in 12% and 24% of tested gulls, respectively. Results from virus microneutralization (MN) tests for antibodies to H1-H12, H14, and H15 varied among years but the highest prevalence of neutralizing antibodies was detected against H1 (24%), H5 (25%), H6 (35%), H9 (33%), and H11 (42%) IAV. The subtype diversity identified by serology in gulls was dominated by Group 1 HA subtypes and only partially reflected the diversity of IAV subtypes isolated from shorebirds.
KEYWORDS:
Leucophaeus atricilla microneutralization; antibodies; influenza A virus; laughing gull; serology
PMID: 27309077 [PubMed - in process]