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J Vet Sci. 2016 Mar;17(1):71-8. doi: 10.4142/jvs.2016.17.1.71. Epub 2016 Mar 22.
A novel M2e-multiple antigenic peptide providing heterologous protection in mice.
Wen F1, Ma JH1, Yu H1, Yang FR1, Huang M1, Zhou YJ1, Li ZJ1, Wang XH1, Li GX1, Jiang YF1, Tong W1, Tong GZ1.
Author information
Abstract
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
KEYWORDS:
H3N2; M2e-multiple antigenic peptide; high-yield; inactivated vaccine; swine influenza virus
PMID: 27051342 [PubMed - in process] PMCID: PMC4808646 Free PMC Article
A novel M2e-multiple antigenic peptide providing heterologous protection in mice.
Wen F1, Ma JH1, Yu H1, Yang FR1, Huang M1, Zhou YJ1, Li ZJ1, Wang XH1, Li GX1, Jiang YF1, Tong W1, Tong GZ1.
Author information
Abstract
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
KEYWORDS:
H3N2; M2e-multiple antigenic peptide; high-yield; inactivated vaccine; swine influenza virus
PMID: 27051342 [PubMed - in process] PMCID: PMC4808646 Free PMC Article