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J Virol Methods. 2015 Apr 17. pii: S0166-0934(15)00142-1. doi: 10.1016/j.jviromet.2015.04.006. [Epub ahead of print]
H5 avian influenza virus pathotyping using oligonucleotide microarray.
Wang LC1, Huang D2, Cheng MC3, Lee SH4, Wang CH5.
Author information
Abstract
The H5 avian influenza virus subtype has huge impact on the poultry industry. Rapid diagnosis and accurate identification of the highly pathogenic avian influenza virus and low-pathogenicity avian influenza virus is essential, especially during H5 outbreaks and surveillance. To this end, a novel and rapid strategy for H5 virus molecular pathotyping is presented. The specific hemagglutinin gene of the H5 virus and the basic amino acid number of the motif at the hemmagglutinin precursor protein cleavage site were detected using oligonucleotide microarray. Highly pathogenic and low-pathogenicity avian influenza viruses in Taiwan were differentiated using 13 microarray probes with the naked eyes. The detection limit reached 3.4 viral RNA copies, 1,000 times more sensitive than reverse transcription polymerase chain reaction. Thus, the oligonucleotide microarray would provide an alternative H5 pathogenicity determination using the naked eye for laboratories lacking facilities.
Copyright ? 2015. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus; Oligonucleotide microarray; Pathotyping
PMID: 25896189 [PubMed - as supplied by publisher]
H5 avian influenza virus pathotyping using oligonucleotide microarray.
Wang LC1, Huang D2, Cheng MC3, Lee SH4, Wang CH5.
Author information
Abstract
The H5 avian influenza virus subtype has huge impact on the poultry industry. Rapid diagnosis and accurate identification of the highly pathogenic avian influenza virus and low-pathogenicity avian influenza virus is essential, especially during H5 outbreaks and surveillance. To this end, a novel and rapid strategy for H5 virus molecular pathotyping is presented. The specific hemagglutinin gene of the H5 virus and the basic amino acid number of the motif at the hemmagglutinin precursor protein cleavage site were detected using oligonucleotide microarray. Highly pathogenic and low-pathogenicity avian influenza viruses in Taiwan were differentiated using 13 microarray probes with the naked eyes. The detection limit reached 3.4 viral RNA copies, 1,000 times more sensitive than reverse transcription polymerase chain reaction. Thus, the oligonucleotide microarray would provide an alternative H5 pathogenicity determination using the naked eye for laboratories lacking facilities.
Copyright ? 2015. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus; Oligonucleotide microarray; Pathotyping
PMID: 25896189 [PubMed - as supplied by publisher]