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J Virol Methods. 2015 Mar 25. pii: S0166-0934(15)00103-2. doi: 10.1016/j.jviromet.2015.03.013. [Epub ahead of print]
Development and Evaluation of a Polydiacetylene Based Biosensor for the Detection of H5 Influenza Virus.
Luo J1, Dong W2, Wang C1, Wen J1, Xia Y2, Wang H1, Ding H3, Jiang L2, He H4.
Author information
Abstract
H5N1 avian influenza has caused serious economic losses as well as posed significant threats to public health, agriculture and wildlife. It is important to develop a rapid, sensitive and specific detection platform suitable for disease surveillance and control. In this study, a highly sensitive, specific and rapid biosensor based on polydiacetylene was developed for detecting H5 influenza virus. The polydiacetylene based biosensor was produced from an optimized ratio of 10,12-Pentacosadiynoic acid and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine, with the anti-H5 influenza antibody embedded onto the vesicle surface. The optimized polydiacetylene vesicle could detect H5 influenza virus sensitively with a detection limit of 0.53 copies per μl, showing a dramatic blue-to-red color change that can be observed directly by the naked eye and recorded by a UV-vis spectrometer. The sensitivity, specificity and accuracy of the biosensor were also evaluated. The sensor could specifically differentiate H5 influenza virus from H3 influenza virus, Newcastle disease virus and porcine reproductive and respiratory syndrome virus. Detection using tracheal swabs was in accord with virus isolation results, and comparable to the RT-PCR method. These results offer the possibility and potential of simple polydiacetylene based bio-analytical method for influenza surveillance.
Copyright ? 2015. Published by Elsevier B.V.
KEYWORDS:
H5 influenza virus; Polydiacetylene; biosensor; chromatic immunoassay; detection; tracheal swabs.
PMID: 25819686 [PubMed - as supplied by publisher]
Development and Evaluation of a Polydiacetylene Based Biosensor for the Detection of H5 Influenza Virus.
Luo J1, Dong W2, Wang C1, Wen J1, Xia Y2, Wang H1, Ding H3, Jiang L2, He H4.
Author information
Abstract
H5N1 avian influenza has caused serious economic losses as well as posed significant threats to public health, agriculture and wildlife. It is important to develop a rapid, sensitive and specific detection platform suitable for disease surveillance and control. In this study, a highly sensitive, specific and rapid biosensor based on polydiacetylene was developed for detecting H5 influenza virus. The polydiacetylene based biosensor was produced from an optimized ratio of 10,12-Pentacosadiynoic acid and 1,2-Dimyristoyl-sn-glycero-3-phosphocholine, with the anti-H5 influenza antibody embedded onto the vesicle surface. The optimized polydiacetylene vesicle could detect H5 influenza virus sensitively with a detection limit of 0.53 copies per μl, showing a dramatic blue-to-red color change that can be observed directly by the naked eye and recorded by a UV-vis spectrometer. The sensitivity, specificity and accuracy of the biosensor were also evaluated. The sensor could specifically differentiate H5 influenza virus from H3 influenza virus, Newcastle disease virus and porcine reproductive and respiratory syndrome virus. Detection using tracheal swabs was in accord with virus isolation results, and comparable to the RT-PCR method. These results offer the possibility and potential of simple polydiacetylene based bio-analytical method for influenza surveillance.
Copyright ? 2015. Published by Elsevier B.V.
KEYWORDS:
H5 influenza virus; Polydiacetylene; biosensor; chromatic immunoassay; detection; tracheal swabs.
PMID: 25819686 [PubMed - as supplied by publisher]