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Infect Genet Evol. 2014 Nov 25. pii: S1567-1348(14)00437-7. doi: 10.1016/j.meegid.2014.11.020. [Epub ahead of print]
Phylogenetic and pathogenic analyses of three H5N1 avian influenza viruses (Clade 2.3.2.1) isolated from wild birds in Northeast China.
Fan Z1, Ci Y2, Liu L2, Ma Y3, Jia Y2, Wang D2, Guan Y2, Tian G2, Ma J4, Li Y5, Chen H2.
Author information
Abstract
From April to September 2012, periodic surveillance of avian influenza H5N1 viruses from different wild bird species was conducted in Northeast China. Three highly pathogenic avian influenza (HPAI) H5N1 viruses were isolated from a yellow-browed warbler, common shoveler, and mallard. To trace the genetic lineage of the isolates, nucleotide sequences of all eight gene segments were determined and phylogenetically analyzed. The data indicated that three viruses belonged to the same antigenic virus group: clade 2.3.2.1. To investigate the pathogenicity of these three viruses in different hosts, chickens, ducks, and mice were inoculated. The results showed that chickens were susceptible to each of the three HPAI H5N1 viruses, resulting in 100% mortality within 2-6 days after infection, whereas the three isolates exhibited distinctly different virulence in ducks and mice. The results of this study demonstrated that HPAI H5N1 viruses of clade2.3.2.1 are still circulating in wild birds through overlapping migratory flyways. Therefore, continuous monitoring of H5N1 in both domestic and wild birds is necessary to prevent a potentially wider outbreak.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus; H5N1; Northeast China; Phylogenetic analysis; Wild bird
PMID:
25461692
[PubMed - as supplied by publisher]
Phylogenetic and pathogenic analyses of three H5N1 avian influenza viruses (Clade 2.3.2.1) isolated from wild birds in Northeast China.
Fan Z1, Ci Y2, Liu L2, Ma Y3, Jia Y2, Wang D2, Guan Y2, Tian G2, Ma J4, Li Y5, Chen H2.
Author information
Abstract
From April to September 2012, periodic surveillance of avian influenza H5N1 viruses from different wild bird species was conducted in Northeast China. Three highly pathogenic avian influenza (HPAI) H5N1 viruses were isolated from a yellow-browed warbler, common shoveler, and mallard. To trace the genetic lineage of the isolates, nucleotide sequences of all eight gene segments were determined and phylogenetically analyzed. The data indicated that three viruses belonged to the same antigenic virus group: clade 2.3.2.1. To investigate the pathogenicity of these three viruses in different hosts, chickens, ducks, and mice were inoculated. The results showed that chickens were susceptible to each of the three HPAI H5N1 viruses, resulting in 100% mortality within 2-6 days after infection, whereas the three isolates exhibited distinctly different virulence in ducks and mice. The results of this study demonstrated that HPAI H5N1 viruses of clade2.3.2.1 are still circulating in wild birds through overlapping migratory flyways. Therefore, continuous monitoring of H5N1 in both domestic and wild birds is necessary to prevent a potentially wider outbreak.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Avian influenza virus; H5N1; Northeast China; Phylogenetic analysis; Wild bird
PMID:
25461692
[PubMed - as supplied by publisher]
