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Methods Mol Biol. 2014;1161:105-18. doi: 10.1007/978-1-4939-0758-8_10.
Avian influenza virus detection and quantitation by real-time rt-PCR.
Spackman E.
Author information
Abstract
Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s for routine surveillance, outbreak assessment and for research. Some of the advantages of rRT-PCR are: high sensitivity, high specificity, rapid time-to-result, scalability, cost, and its inherently quantitative nature. Furthermore, rRT-PCR can be used with numerous sample types, is less expensive than virus isolation in chicken embryos, and since infectious virus is inactivated early during processing, biosafety and bio-security are also easier to maintain. However, the high genetic variability of AIV may decrease sensitivity and increases the chances of a false negative result. This chapter provides an overview of the USDA validated rRT-PCR procedure for the detection of type A influenza.
PMID:
24899424
[PubMed - in process]
Avian influenza virus detection and quantitation by real-time rt-PCR.
Spackman E.
Author information
Abstract
Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s for routine surveillance, outbreak assessment and for research. Some of the advantages of rRT-PCR are: high sensitivity, high specificity, rapid time-to-result, scalability, cost, and its inherently quantitative nature. Furthermore, rRT-PCR can be used with numerous sample types, is less expensive than virus isolation in chicken embryos, and since infectious virus is inactivated early during processing, biosafety and bio-security are also easier to maintain. However, the high genetic variability of AIV may decrease sensitivity and increases the chances of a false negative result. This chapter provides an overview of the USDA validated rRT-PCR procedure for the detection of type A influenza.
PMID:
24899424
[PubMed - in process]
