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Methods Mol Biol. 2014;1161:151-67. doi: 10.1007/978-1-4939-0758-8_14.
Detection of influenza a antibodies in avian serum samples by ELISA.
Chappell L1, Killian ML, Spackman E.
Author information
Abstract
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to-use assay with good quality control. Various sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation, and troubleshooting are discussed.
PMID:
24899428
[PubMed - in process]
Detection of influenza a antibodies in avian serum samples by ELISA.
Chappell L1, Killian ML, Spackman E.
Author information
Abstract
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive, they provide a ready-to-use assay with good quality control. Various sample types can be processed for ELISA: serum, plasma, egg yolk, blood collected on filter paper. Quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, results interpretation, and troubleshooting are discussed.
PMID:
24899428
[PubMed - in process]
