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Euro Surveill. Missed diagnosis of influenza B virus due to nucleoprotein sequence mutations, Singapore, April 2011
[Source: Eurosurveillance, full text: (LINK). Abstract, edited.]
Eurosurveillance, Volume 16, Issue 33, 18 August 2011
Rapid communications
Missed diagnosis of influenza B virus due to nucleoprotein sequence mutations, Singapore, April 2011
H K Lee<SUP>1</SUP>, C K Lee<SUP>1</SUP>, T P Loh<SUP>2</SUP>, D Chiang<SUP>3</SUP>, E S Koay<SUP>1</SUP><SUP>,4</SUP>, J W Tang (
)<SUP>1</SUP>
Citation style for this article: Lee HK, Lee CK, Loh TP, Chiang D, Koay ES, Tang JW. Missed diagnosis of influenza B virus due to nucleoprotein sequence mutations, Singapore, April 2011 . Euro Surveill. 2011;16(33):pii=19943. Available online: http://www.eurosurveillance.org/View...rticleId=19943
Date of submission: 08 August 2011 <HR>A new influenza B variant was discovered in Singapore in April 2011 during diagnostic testing of a 3-year-old boy with respiratory symptoms. Influenza B virus was isolated from culture and confirmed by standard immunofluorescence testing, but was not detected by the routine, in-house influenza screening reverse-transcription polymerase chain reaction assay that targets the nucleoprotein (NP) gene. Subsequent sequencing investigations demonstrated that several other published assays targeting NP could also fail to detect this novel variant.
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Eurosurveillance, Volume 16, Issue 33, 18 August 2011
Rapid communications
Missed diagnosis of influenza B virus due to nucleoprotein sequence mutations, Singapore, April 2011
H K Lee<SUP>1</SUP>, C K Lee<SUP>1</SUP>, T P Loh<SUP>2</SUP>, D Chiang<SUP>3</SUP>, E S Koay<SUP>1</SUP><SUP>,4</SUP>, J W Tang (
- Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Hospital, Singapore
- Clinical Chemistry Division, Department of Laboratory Medicine, National University Hospital, Singapore
- Microbiology Division, Department of Laboratory Medicine, National University Hospital, Singapore
- Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Citation style for this article: Lee HK, Lee CK, Loh TP, Chiang D, Koay ES, Tang JW. Missed diagnosis of influenza B virus due to nucleoprotein sequence mutations, Singapore, April 2011 . Euro Surveill. 2011;16(33):pii=19943. Available online: http://www.eurosurveillance.org/View...rticleId=19943
Date of submission: 08 August 2011 <HR>A new influenza B variant was discovered in Singapore in April 2011 during diagnostic testing of a 3-year-old boy with respiratory symptoms. Influenza B virus was isolated from culture and confirmed by standard immunofluorescence testing, but was not detected by the routine, in-house influenza screening reverse-transcription polymerase chain reaction assay that targets the nucleoprotein (NP) gene. Subsequent sequencing investigations demonstrated that several other published assays targeting NP could also fail to detect this novel variant.