First French draft genome sequence of Monkeypox virus, may 2022 - virological.org

[Mary Wilson]

May 26 2022

Guillaume Croville ¹, Mathilda Walch¹, Jean-Luc Guérin¹, Jean-Michel Mansuy², Christophe Pasquier², Jacques Izopet²

A full Monkeypox virus genome was obtained from a male patient sampled at the University Hospital Center of Toulouse Purpan (France) in May 2022. We applied an Oxford Nanopore Technologies sequencing method directly on the clinical material as already done before by our team for the sequencing of an avipoxvirus ^a. ...

https://virological.org/t/first-french-draft-genome-sequence-of-monkeypox-virus-may-2022/819

[Mary Wilson]

Illumina whole-genome sequence of Monkeypox virus in a patient travelling from the Canary Islands to France - Genome Reports

May 28 2022
(updated) 5 hr ago

Gregory Destras^1,2,3, Antonin Bal^1,2,3, Bruno Simon^1,2, Quentin Semanas^1,2, Hadrien Règue^1,2, Bruno Lina^1,2,3, Laurence Josset^1,2,3

Whole-genome sequencing (WGS) of the first cases of Monkeypox virus responsible of the 2022 outbreak in Europe has shown that sequences were highly similar and belonged to the West African clade [https://virological.org/t/first-monkeypox-genome-sequence-from-the-netherlands/821; 36 Multi-country outbreak of Monkeypox virus: genetic divergence and first signs of microevolution 23]. Most of these sequences were initially performed using ONT. Obtaining Monkeypox virus sequences from different platform technologies is crucial to avoid sequencing errors.

Here we report the first french Monkeypox whole-genome sequence performed on Illumina platform, from a male patient returning from Canary Islands on 19 May 2022. Skin lesions were sampled on 22 May 2022 at Emergency Unit of Hospices Civils de Lyon, France. ...

https://virological.org/t/illumina-whole-genome-sequence-of-monkeypox-virus-in-a-patient-travelling-from-the-canary-islands-to-france/829