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May 27 2022
Alessia Lai 1, Annalisa Bergna 1, Carla Della Ventura 1, Maciej Tarkowski 1, Agostino Riva 1,2, Davide Moschese 3, Giuliano Rizzardini 3, Spinello Antinori 1,2, Gianguglielmo Zehender 1
... The virus is transmitted from one person to another by close contact with lesions, body fluids, respiratory droplets and contaminated materials. The Monkeypox lethality rate ranges from 1 to 10% (2), with highest proportion in children. MPXV forms two distinct clades: the MPXV Congo Basin clade viruses that are endemic in the Congo Basin and the MPXV West African clade viruses that have been isolated in West Africa and appear to cause a less severe, and less interhuman transmissible disease (3).
... Here we report the full genome of an Italian case obtained from a swab collected on May 25th from skin lesions of a male patient.
On May 24 a 33-yr old man presented to our ward with a perianal ulcer, inguinal bilateral adenopathy and few scattered papular and vesicular skin lesions localized on both elbows, back, buttock and left foot. He lived in Lisbon (Portugal) and arrived in Italy on May 18th. A few days before the appearance of skin lesions he complained of fever (38.5°C) of one-day duration associated with pharyngodynia and sneezing.
Brief description of the methods
DNA was extracted from all samples using the QIAamp DNA Blood kit (QIAGEN, Hilden, Germany), fragmented using a Covaris M220 ultrasonicator (Covaris, Woburn, MA, USA) and checked using a 4200 TapeStation System (Agilent Technologies, USA). The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) and sequencing was performed using a Miseq sequencer (Illumina) with 300 cycles.
... The Italian sequence is depicted in red. The sequence has been submitted to GenBank and the accession number will be posted when available (within two working days)
(Note: Click on diagrams)
Alessia Lai 1, Annalisa Bergna 1, Carla Della Ventura 1, Maciej Tarkowski 1, Agostino Riva 1,2, Davide Moschese 3, Giuliano Rizzardini 3, Spinello Antinori 1,2, Gianguglielmo Zehender 1
... The virus is transmitted from one person to another by close contact with lesions, body fluids, respiratory droplets and contaminated materials. The Monkeypox lethality rate ranges from 1 to 10% (2), with highest proportion in children. MPXV forms two distinct clades: the MPXV Congo Basin clade viruses that are endemic in the Congo Basin and the MPXV West African clade viruses that have been isolated in West Africa and appear to cause a less severe, and less interhuman transmissible disease (3).
... Here we report the full genome of an Italian case obtained from a swab collected on May 25th from skin lesions of a male patient.
On May 24 a 33-yr old man presented to our ward with a perianal ulcer, inguinal bilateral adenopathy and few scattered papular and vesicular skin lesions localized on both elbows, back, buttock and left foot. He lived in Lisbon (Portugal) and arrived in Italy on May 18th. A few days before the appearance of skin lesions he complained of fever (38.5°C) of one-day duration associated with pharyngodynia and sneezing.
Brief description of the methods
DNA was extracted from all samples using the QIAamp DNA Blood kit (QIAGEN, Hilden, Germany), fragmented using a Covaris M220 ultrasonicator (Covaris, Woburn, MA, USA) and checked using a 4200 TapeStation System (Agilent Technologies, USA). The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) and sequencing was performed using a Miseq sequencer (Illumina) with 300 cycles.
... The Italian sequence is depicted in red. The sequence has been submitted to GenBank and the accession number will be posted when available (within two working days)
(Note: Click on diagrams)