May 23 2022

Bert Vanmechelen, Tony Wawina-Bokalanga and Piet Maes

A Monkeypox virus genome was obtained from a second Belgian case at the Rega Institute, KU Leuven. Viral DNA was extracted from each swab sample (three in total) using the QIAamp DNA Mini Kit (QIAGEN) according to the manufacturer’s instructions. Subsequently, a conventional PCR was performed with Monkeypox-specific primers, confirming the presence of Monkeypox virus DNA in each extract. ...

A Monkeypox virus genome from a second Belgian case
https://virological.org/t/a-monkeypox-virus-genome-from-a-second-belgian-case/807/1
Bert Vanmechelen, Tony Wawina-Bokalanga and Piet Maes. A Monkeypox virus genome was obtained from a second Belgian case at the Rega Institute, KU Leuven. Viral DNA was extracted from each swab sample (three in total) using the QIAamp DNA Mini Kit (QIAGEN) according to the manufacturer’s instructions. Subsequently, a conventional PCR was performed with Monkeypox-specific primers, confirming the presence of Monkeypox virus DNA in each extract. Sequencing libraries were prepared using the ligatio...