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Cell Transplant. 2012 Sep 21. [Epub ahead of print]
Influenza virus infects bone marrow mesenchymal stromal cells in vitro: implications for bone marrow transplantation.
Khatri M, Saif YM.
Abstract
Mesenchymal stromal cells (MSCs) have differentiation, immunomodulatory and self-renewal properties and are, therefore, an attractive tool for regenerative medicine and autoimmune diseases. MSCs may be of great value to treat graft-versus-host disease. Influenza virus causes highly contagious seasonal infection and occasional pandemics. The infection is severe in children, elderly and immunocompromised hosts including hematopoietic stem cell transplant patients. The objective of this study was to determine if MSCs are permissive to influenza virus replication. We isolated MSCs from the bone marrow of 4-6 week-old germ-free pigs. Swine and human influenza virus strains were used to infect MSCs in vitro. MSCs expressed known influenza virus alpha-2,3 and alpha-2,6 sialic acid receptors and supported replication of swine and human influenza viruses. Viral infection of MSCs resulted in cell lysis and proinflammatory cytokine production. These findings demonstrate that bone-marrow derivedMSCs are susceptible to influenza virus. The data also suggests that transplantation of bone marrow MSCs from influenza virus-infected donors may transmit infection to recipients.Also, MSCs may get infected if infused into a patient with an ongoing influenza virus infection.
PMID:
23006541
[PubMed - as supplied by publisher]
Influenza virus infects bone marrow mesenchymal stromal cells in vitro: implications for bone marrow transplantation.
Khatri M, Saif YM.
Abstract
Mesenchymal stromal cells (MSCs) have differentiation, immunomodulatory and self-renewal properties and are, therefore, an attractive tool for regenerative medicine and autoimmune diseases. MSCs may be of great value to treat graft-versus-host disease. Influenza virus causes highly contagious seasonal infection and occasional pandemics. The infection is severe in children, elderly and immunocompromised hosts including hematopoietic stem cell transplant patients. The objective of this study was to determine if MSCs are permissive to influenza virus replication. We isolated MSCs from the bone marrow of 4-6 week-old germ-free pigs. Swine and human influenza virus strains were used to infect MSCs in vitro. MSCs expressed known influenza virus alpha-2,3 and alpha-2,6 sialic acid receptors and supported replication of swine and human influenza viruses. Viral infection of MSCs resulted in cell lysis and proinflammatory cytokine production. These findings demonstrate that bone-marrow derivedMSCs are susceptible to influenza virus. The data also suggests that transplantation of bone marrow MSCs from influenza virus-infected donors may transmit infection to recipients.Also, MSCs may get infected if infused into a patient with an ongoing influenza virus infection.
PMID:
23006541
[PubMed - as supplied by publisher]
