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Microbes Infect
. 2023 Nov 17:105252.
doi: 10.1016/j.micinf.2023.105252. Online ahead of print. Middle East respiratory coronavirus (MERS-CoV) internalized by llama alveolar macrophages does not result in virus replication or induction of pro-inflammatory cytokines
Jordi Rodon 1 , Martin Sachse 2 , Nigeer Te 3 , Joaquim Segalés 4 , Albert Bensaid 5 , Cristina Risco 6 , Júlia Vergara-Alert 7
Affiliations
Severe Middle East respiratory syndrome (MERS) is characterized by massive infiltration of immune cells in lungs. MERS-coronavirus (MERS-CoV) replicates in vitro in human macrophages, inducing high pro-inflammatory responses. In contrast, camelids, the main reservoir for MERS-CoV, are asymptomatic carriers. Although limited infiltration of leukocytes has been observed in the lower respiratory tract of camelids, their role during infection remains unknown. Here we studied whether llama alveolar macrophages (LAMs) are susceptible to MERS-CoV infection and can elicit pro-inflammatory responses. MERS-CoV did not replicate in LAMs; however, they effectively capture and degrade viral particles. Moreover, transcriptomic analyses showed that LAMs do not induce pro-inflammatory cytokines upon MERS-CoV sensing.
Keywords: Alveolar macrophages; MERS-CoV; Middle East respiratory syndrome coronavirus; immune responses; llama; pro-inflammatory cytokines.
. 2023 Nov 17:105252.
doi: 10.1016/j.micinf.2023.105252. Online ahead of print. Middle East respiratory coronavirus (MERS-CoV) internalized by llama alveolar macrophages does not result in virus replication or induction of pro-inflammatory cytokines
Jordi Rodon 1 , Martin Sachse 2 , Nigeer Te 3 , Joaquim Segalés 4 , Albert Bensaid 5 , Cristina Risco 6 , Júlia Vergara-Alert 7
Affiliations
- PMID: 37981029
- DOI: 10.1016/j.micinf.2023.105252
Severe Middle East respiratory syndrome (MERS) is characterized by massive infiltration of immune cells in lungs. MERS-coronavirus (MERS-CoV) replicates in vitro in human macrophages, inducing high pro-inflammatory responses. In contrast, camelids, the main reservoir for MERS-CoV, are asymptomatic carriers. Although limited infiltration of leukocytes has been observed in the lower respiratory tract of camelids, their role during infection remains unknown. Here we studied whether llama alveolar macrophages (LAMs) are susceptible to MERS-CoV infection and can elicit pro-inflammatory responses. MERS-CoV did not replicate in LAMs; however, they effectively capture and degrade viral particles. Moreover, transcriptomic analyses showed that LAMs do not induce pro-inflammatory cytokines upon MERS-CoV sensing.
Keywords: Alveolar macrophages; MERS-CoV; Middle East respiratory syndrome coronavirus; immune responses; llama; pro-inflammatory cytokines.